Cytokine profile of human CD4+ T lymphocytes following CD3/CD28 or CD3/CD46 stimulation
. | Activation condition . | . | . | ||
|---|---|---|---|---|---|
| Cytokines, pg/mL . | CD3 . | CD3/CD28 . | CD3/CD46 . | ||
| IL-1α* | — | 30 ± 1.5 | 20 ± 1.0 | ||
| IL-1β* | — | — | — | ||
| IL-2* | — | 500 ± 100 | — | ||
| IL-4* | — | 50 ± 2.0 | — | ||
| IL-6* | — | — | — | ||
| IL-7* | — | — | — | ||
| IL-8* | 30 ± 3.0 | 100 ± 15 | 350 ± 65 | ||
| IL-10* | — | 250 ± 50 | 1800 ± 300‡ | ||
| IL-12† | — | — | — | ||
| IL-15† | 40 ± 2 | 70 ± 2 | 20 ± 2.0 | ||
| TNF-α* | 50 ± 8.0 | 200 ± 30 | 600 ± 100‡ | ||
| IFN-γ* | 80 ± 14 | 200 ± 40 | 900 ± 150‡ | ||
| GM-CSF* | 100 ± 10 | 300 ± 40 | 2200 ± 400‡ | ||
| Soluble CD40L (CD154)† | — | 80 ± 5 | 1300 ± 190‡ | ||
. | Activation condition . | . | . | ||
|---|---|---|---|---|---|
| Cytokines, pg/mL . | CD3 . | CD3/CD28 . | CD3/CD46 . | ||
| IL-1α* | — | 30 ± 1.5 | 20 ± 1.0 | ||
| IL-1β* | — | — | — | ||
| IL-2* | — | 500 ± 100 | — | ||
| IL-4* | — | 50 ± 2.0 | — | ||
| IL-6* | — | — | — | ||
| IL-7* | — | — | — | ||
| IL-8* | 30 ± 3.0 | 100 ± 15 | 350 ± 65 | ||
| IL-10* | — | 250 ± 50 | 1800 ± 300‡ | ||
| IL-12† | — | — | — | ||
| IL-15† | 40 ± 2 | 70 ± 2 | 20 ± 2.0 | ||
| TNF-α* | 50 ± 8.0 | 200 ± 30 | 600 ± 100‡ | ||
| IFN-γ* | 80 ± 14 | 200 ± 40 | 900 ± 150‡ | ||
| GM-CSF* | 100 ± 10 | 300 ± 40 | 2200 ± 400‡ | ||
| Soluble CD40L (CD154)† | — | 80 ± 5 | 1300 ± 190‡ | ||
Purified peripheral T cells (CD4+/CD45RA+) were activated with the indicated antibodies, supernatants were collected at day 3, and secreted cytokines were measured using the *ProteoPlexTM 16-Well Human Cytokine Array (Novagen, Madison, WI) or †ELISA. The expression of IL-10, TNF-α, IFN-γ, and GM-CSF was also confirmed by ELISA (not shown). Activation conditions were performed in triplicate. Data are the mean ± SD of 5 independent experiments using 5 different donors.
— indicates not detectable (cytokine content < 5 pg/mL)
The observed level of statistical difference in cytokine production between CD3/CD28- and CD3/CD46-activated cells was P < .001 by the paired Student t test in all cases