Alanine scanning of the TpoR KWQFP juxtamembrane motif
wtTpoR | KWQFPAHYRRLRHALWPSLPDL |
| TpoR-AAQFP | AAQFPAHYRRLRHALWPSLPDL |
| TpoR-AWQFP | AWQFPAHYRRLRHALWPSLPDL |
| TpoR-KAQFP | KAQFPAHYRRLRHALWPSLPDL |
| TpoR-KWAAP | KWAAPAHYRRLRHALWPSLPDL |
| TpoR-KWQFA | KWQFAAHYRRLRHALWPSLPDL |
wtTpoR | KWQFPAHYRRLRHALWPSLPDL |
| TpoR-AAQFP | AAQFPAHYRRLRHALWPSLPDL |
| TpoR-AWQFP | AWQFPAHYRRLRHALWPSLPDL |
| TpoR-KAQFP | KAQFPAHYRRLRHALWPSLPDL |
| TpoR-KWAAP | KWAAPAHYRRLRHALWPSLPDL |
| TpoR-KWQFA | KWQFAAHYRRLRHALWPSLPDL |
The 5 JM amino acid residues of the TpoR were substituted by alanines, single or in pairs. Underlined are the KWQFP motif and the locations of the substituted alanines.