Presence of p540-specific CD8+ T cells and CTLs in HLA-A2+ cancer patients
Patient no. . | Frequency of tetramer-positive CD8+ cells in PBMCs . | Frequency of p540-specific CTLp's in PBMCs . | Ability to generate a p540-specific CTL line . |
|---|---|---|---|
| 1 | 1:1538 | 1 : 5 082 | + |
| 2 | 1:1960 | 1 : 2 596 | + |
| 3 | 1:1428 | < 1 : 10 000 | – |
| 4 | ND | ND | + |
| 5 | ND | ND | + |
| 6 | 1:250 | 1 : 1 438 | + |
| 7 | 0 | 0 | – |
| 8 | 1:3250 | < 1 : 10 000 | – |
| 9 | 1:2500 | 1 : 7 123 | – |
| 10 | ND | 1 : 1 443 | ND |
| 11 | 1:833 | 1 : 298 | + |
| 12 | 1:2700 | 1 : 6 343 | ND |
| 13 | 1:1000 | 1 : 1 684 | ND |
| 14 | 1:1000 | 1 : 540 | ND |
| 15 | 1:1666 | 1 : 354 | ND |
| 16 | ND | ND | + |
| 17 | 1:1000 | 1 : 2 976 | ND |
| 18 | 1:500 | 1 : 1 298 | + |
| 21 | 0 | 0 | – |
| 22 | ND | ND | + |
| 23 | ND | ND | + |
| 26 | 1:3500 | < 1 : 10 000 | ND |
| 27 | 1:1428 | ND | + |
Patient no. . | Frequency of tetramer-positive CD8+ cells in PBMCs . | Frequency of p540-specific CTLp's in PBMCs . | Ability to generate a p540-specific CTL line . |
|---|---|---|---|
| 1 | 1:1538 | 1 : 5 082 | + |
| 2 | 1:1960 | 1 : 2 596 | + |
| 3 | 1:1428 | < 1 : 10 000 | – |
| 4 | ND | ND | + |
| 5 | ND | ND | + |
| 6 | 1:250 | 1 : 1 438 | + |
| 7 | 0 | 0 | – |
| 8 | 1:3250 | < 1 : 10 000 | – |
| 9 | 1:2500 | 1 : 7 123 | – |
| 10 | ND | 1 : 1 443 | ND |
| 11 | 1:833 | 1 : 298 | + |
| 12 | 1:2700 | 1 : 6 343 | ND |
| 13 | 1:1000 | 1 : 1 684 | ND |
| 14 | 1:1000 | 1 : 540 | ND |
| 15 | 1:1666 | 1 : 354 | ND |
| 16 | ND | ND | + |
| 17 | 1:1000 | 1 : 2 976 | ND |
| 18 | 1:500 | 1 : 1 298 | + |
| 21 | 0 | 0 | – |
| 22 | ND | ND | + |
| 23 | ND | ND | + |
| 26 | 1:3500 | < 1 : 10 000 | ND |
| 27 | 1:1428 | ND | + |
ND indicates not done; +, successful CTL line generation; and –, lack of CTL line generation.