Table 3. Higher AAV neutralizing... | American Society of Hematology

Table 3.

Higher AAV neutralizing capacity detected in vivo than in vitro

		Percent of 5 × 10¹¹ vg of AAV-hFIX neutralized in vivo†
Neutralizing antibody titer	Neutralizing capacity in vitro,^*vg	Predicted‡	Actual^§
1:3.3	4.5 × 10¹⁰	4.5	100
1:10	9 × 10¹⁰	9	100

		Percent of 5 × 10¹¹ vg of AAV-hFIX neutralized in vivo†
Neutralizing antibody titer	Neutralizing capacity in vitro,^*vg	Predicted‡	Actual^§
1:3.3	4.5 × 10¹⁰	4.5	100
1:10	9 × 10¹⁰	9	100

Determined by in vitro neutralizing capacity assay as described in Materials and methods. The neutralizing capacity of a specified titer was defined as the maximal vector dose neutralized by 50% or less

†

Determined in SCID mice reconstituted with IVIG and intravenously administered vector

‡

The predicted percentage was derived by dividing 50% of in vitro neutralizing capacity (vg) by the injected vector dose of 5 × 10¹¹ vg in vivo

As observed in vivo

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