Soluble factors secreted by MCTSs with or without monocyte coculture
. | M-CSF, pg/mL* . | IL-6, pg/mL . | Lactate, mg/dL . |
|---|---|---|---|
| MelIm MCTSs† | 34 ± 3 | < 3 | 98 ± 12 (10.8 mM) |
| MelIm MCTSs and monocytes | 171 ± 40 | 23 ± 4 | ND |
| J82 MCTSs | 1367 ± 289 | 12 150 ± 488 | 18 ± 2 (2 mM) |
| J82 MCTSs and monocytes | 583 ± 167 | 9 241 ± 1894 | ND |
. | M-CSF, pg/mL* . | IL-6, pg/mL . | Lactate, mg/dL . |
|---|---|---|---|
| MelIm MCTSs† | 34 ± 3 | < 3 | 98 ± 12 (10.8 mM) |
| MelIm MCTSs and monocytes | 171 ± 40 | 23 ± 4 | ND |
| J82 MCTSs | 1367 ± 289 | 12 150 ± 488 | 18 ± 2 (2 mM) |
| J82 MCTSs and monocytes | 583 ± 167 | 9 241 ± 1894 | ND |
Data represent the mean ± SEM of at least 3 independent experiments with different monocyte donors.
ND indicates not determined.
IL-6 and M-CSF were determined by ELISA. Lactate was measured with a clinical routine procedure
MCTSs were cultured with or without monocytes for 7 days. Supernatants of 20 wells were pooled, filtered, and stored at –20°C