Primers used for cloning of IL-7/HGFβ into expression vectors
Primers . | Primer Sequence (5′-3′) . | Underlined sequences . |
|---|---|---|
| A | GCGCATTCTGCCTTTGCGGAGTGCCACATTAAAGAC | NA |
| B | CGACCCACCACCGCCCGAGCCACCGCCTCCTATACTGCCCTTCAAAAT | Linker |
| C | GGGATCGTCGGTTTTGTA | NA |
| D | CGCAAAGGCAGAATGCGC | NA |
| E | GGAGGCGGTGGCTCGGGCGGTGGTGGGTCGGTTGTAAATGGCATTCCA | Linker |
| F | TGCTCTAGACTATTACAACTTGTATGTCAA | Xbal site |
| G | CGCGGCGCGCCGAGTGCCACATTAAAGAC | Ascl site |
| H | CGCCTCGAGCTATTACAACTTGTATGTCAA | Xhol site |
| I | CGCCTCGAGAAAAGAGAGTGCCACATTAAAGAC | Xhol site |
Primers . | Primer Sequence (5′-3′) . | Underlined sequences . |
|---|---|---|
| A | GCGCATTCTGCCTTTGCGGAGTGCCACATTAAAGAC | NA |
| B | CGACCCACCACCGCCCGAGCCACCGCCTCCTATACTGCCCTTCAAAAT | Linker |
| C | GGGATCGTCGGTTTTGTA | NA |
| D | CGCAAAGGCAGAATGCGC | NA |
| E | GGAGGCGGTGGCTCGGGCGGTGGTGGGTCGGTTGTAAATGGCATTCCA | Linker |
| F | TGCTCTAGACTATTACAACTTGTATGTCAA | Xbal site |
| G | CGCGGCGCGCCGAGTGCCACATTAAAGAC | Ascl site |
| H | CGCCTCGAGCTATTACAACTTGTATGTCAA | Xhol site |
| I | CGCCTCGAGAAAAGAGAGTGCCACATTAAAGAC | Xhol site |
NA indicates not applicable.