Effect of plasma fibronectin or its N-terminal 70K fragment on surface coverage, thrombus volume, and number of adherent platelets at a wall shear rate of 1250 s–1
Substrate and perfusate . | Surface coverage, μm2 × 10-4 (%) . | Thrombus volume, μm3 × 10-4 . | Adherent platelets, × 10-6 . |
|---|---|---|---|
| Fibrin (+FXIII) | 0.3 ± 0.1 (5) | 1.4 ± 0.5 | 2.0 ± 0.8 |
| + FN | 1.9 ± 0.5 (33)* | 6.4 ± 1.2* | 7.8 ± 1.7* |
| + 70K | 0.3 ± 0.1 (5) | 1.7 ± 0.6 | 1.7 ± 0.8 |
| Fibrin (-FXIII) | 0.3 ± 0.1 (5) | 1.5 ± 0.6 | 1.8 ± 0.6 |
| + FN | 1.5 ± 0.7 (26)† | 5.6 ± 1.6† | 6.5 ± 1.9† |
| FN-fibrin (+FXIII) | 1.2 ± 0.3 (20)‡ | 3.5 ± 0.8‡ | 6.0 ± 1.4‡ |
| + FN | 4.2 ± 0.7 (72)*‡ | 11.3 ± 1.5*‡ | 18.0 ± 2.5*‡ |
| + 70K | 1.1 ± 0.3 (19)‡ | 5.0 ± 1.9‡ | 5.6 ± 1.9‡ |
| FN-fibrin (-FXIII) | 0.4 ± 0.1 (6) | 1.7 ± 0.6 | 1.9 ± 0.7 |
| + FN | 1.7 ± 0.6 (29)† | 5.9 ± 1.8† | 6.8 ± 1.6† |
Substrate and perfusate . | Surface coverage, μm2 × 10-4 (%) . | Thrombus volume, μm3 × 10-4 . | Adherent platelets, × 10-6 . |
|---|---|---|---|
| Fibrin (+FXIII) | 0.3 ± 0.1 (5) | 1.4 ± 0.5 | 2.0 ± 0.8 |
| + FN | 1.9 ± 0.5 (33)* | 6.4 ± 1.2* | 7.8 ± 1.7* |
| + 70K | 0.3 ± 0.1 (5) | 1.7 ± 0.6 | 1.7 ± 0.8 |
| Fibrin (-FXIII) | 0.3 ± 0.1 (5) | 1.5 ± 0.6 | 1.8 ± 0.6 |
| + FN | 1.5 ± 0.7 (26)† | 5.6 ± 1.6† | 6.5 ± 1.9† |
| FN-fibrin (+FXIII) | 1.2 ± 0.3 (20)‡ | 3.5 ± 0.8‡ | 6.0 ± 1.4‡ |
| + FN | 4.2 ± 0.7 (72)*‡ | 11.3 ± 1.5*‡ | 18.0 ± 2.5*‡ |
| + 70K | 1.1 ± 0.3 (19)‡ | 5.0 ± 1.9‡ | 5.6 ± 1.9‡ |
| FN-fibrin (-FXIII) | 0.4 ± 0.1 (6) | 1.7 ± 0.6 | 1.9 ± 0.7 |
| + FN | 1.7 ± 0.6 (29)† | 5.9 ± 1.8† | 6.8 ± 1.6† |
The surface coverage and thrombus volume were measured from confocal microscopic images, and the number of adherent platelets was calculated by luminescence signal as described in “Materials and methods.” Values represent the mean ± SD (n = 4-6).
FN indicates fibronectin; 70K, N-terminal 70 kDa region of FN.
P < .01 as compared with the absence of plasma FN on each substrate-coated surface.
n = 2-3 (statistics were not applied).
P < .01 as compared with a fibrin substrate.