Effect of antibody against integrin on platelet accumulation on fibrin or fibronectin-fibrin in the absence of soluble fibronectin at a wall shear rate of 1250s–1
. | Adherent platelets, × 10-6 (% inhibition) . | . | |
|---|---|---|---|
| Antibody . | Fibrin . | FN-fibrin . | |
| Control | 1.8 ± 0.7 | 5.9 ± 1.2 | |
| With mouse IgG1 | 1.7 ± 0.6 (5) | 5.6 ± 1.0 (6) | |
| With rat IgG2 | 1.6 ± 0.7 (9) | 5.3 ± 0.9 (11) | |
| With anti-β3 (7E3) | 0.2 ± 0.1 (90)* | 0.7 ± 0.3 (88)* | |
| With anti-αIIbβ3 (10E5) | 0.2 ± 0.1 (90)* | 0.8 ± 0.3 (87)* | |
| With anti-αvβ3 (LM609) | 1.0 ± 0.5 (47)* | 3.0 ± 1.4 (50)* | |
| With anti-β1 (mAb13) | 1.6 ± 0.9 (12) | 4.6 ± 1.8 (24) | |
. | Adherent platelets, × 10-6 (% inhibition) . | . | |
|---|---|---|---|
| Antibody . | Fibrin . | FN-fibrin . | |
| Control | 1.8 ± 0.7 | 5.9 ± 1.2 | |
| With mouse IgG1 | 1.7 ± 0.6 (5) | 5.6 ± 1.0 (6) | |
| With rat IgG2 | 1.6 ± 0.7 (9) | 5.3 ± 0.9 (11) | |
| With anti-β3 (7E3) | 0.2 ± 0.1 (90)* | 0.7 ± 0.3 (88)* | |
| With anti-αIIbβ3 (10E5) | 0.2 ± 0.1 (90)* | 0.8 ± 0.3 (87)* | |
| With anti-αvβ3 (LM609) | 1.0 ± 0.5 (47)* | 3.0 ± 1.4 (50)* | |
| With anti-β1 (mAb13) | 1.6 ± 0.9 (12) | 4.6 ± 1.8 (24) | |
The luminescence signal was obtained as described in “Materials and methods.” Values represent the mean ± SD of 3 to 4 separate experiments.
P < .01 as compared with a control group.