Summary of results of bone marrow transplantation experiments
Adoptive transfer protocol . | No. of mice receiving transplants . | No. of mice with leukemia . | Survival time, d . | Terminal WBC count, × 109/L . | Spleen total yield, × 107 ± SD . | Leukemia onset, wk . |
|---|---|---|---|---|---|---|
| BALB/c wt | ||||||
| ICN1 | 16 | 16 | 58 | 158.5 | 88.0 ± 8.3 | 2 |
| Empty, killed | 11 | 0 | 58 | 10.2 | 4.5 ± 0.7 | NA |
| Rag2−/− | ||||||
| ICN1 | 13 | 12 | 80 | 27.6 | 23.2 ± 2.6 | 8 |
| ICN1 + 1 × CD3 | 15 | 15 | 77 | 28.1 | 35.7 ± 4.3 | 3 |
| ICN1 + 3 × CD3 | 13 | 13 | 62 | 180.2 | 91.8 ± 11.2 | 3 |
| Empty + 3 × CD3, killed | 12 | 0 | 76 | 9.9 | 1.7 ± 0.2 | NA |
Adoptive transfer protocol . | No. of mice receiving transplants . | No. of mice with leukemia . | Survival time, d . | Terminal WBC count, × 109/L . | Spleen total yield, × 107 ± SD . | Leukemia onset, wk . |
|---|---|---|---|---|---|---|
| BALB/c wt | ||||||
| ICN1 | 16 | 16 | 58 | 158.5 | 88.0 ± 8.3 | 2 |
| Empty, killed | 11 | 0 | 58 | 10.2 | 4.5 ± 0.7 | NA |
| Rag2−/− | ||||||
| ICN1 | 13 | 12 | 80 | 27.6 | 23.2 ± 2.6 | 8 |
| ICN1 + 1 × CD3 | 15 | 15 | 77 | 28.1 | 35.7 ± 4.3 | 3 |
| ICN1 + 3 × CD3 | 13 | 13 | 62 | 180.2 | 91.8 ± 11.2 | 3 |
| Empty + 3 × CD3, killed | 12 | 0 | 76 | 9.9 | 1.7 ± 0.2 | NA |
Retroviruses (ICN1 or empty) and number of anti-CD3 injections (1 ×, single; 3 ×, triple) used in the transplantation assay are indicated. Leukemia onset is defined as time (in weeks) after bone marrow transplantation when EGFP+CD4+CD8+ cells were identified in the peripheral blood.
NA indicates not applicable.