Percentage of different platelet formation stages on wild-type and WASp-deficient bone marrow EM sections
. | Control . | Wasp−/− . |
|---|---|---|
| Maturing MKs with no apparent platelet territories | 52 | 11 |
| MKs exhibiting platelet territories (Figure 2A) | 48 | 34 |
| MKs in the process of shedding (Figure 2B) | 0 | 25 |
| Isolated platelet clusters (Figure 2C) | 0 | 23 |
| Naked nuclei (Figure 2D) | 0 | 7 |
. | Control . | Wasp−/− . |
|---|---|---|
| Maturing MKs with no apparent platelet territories | 52 | 11 |
| MKs exhibiting platelet territories (Figure 2A) | 48 | 34 |
| MKs in the process of shedding (Figure 2B) | 0 | 25 |
| Isolated platelet clusters (Figure 2C) | 0 | 23 |
| Naked nuclei (Figure 2D) | 0 | 7 |
Bone marrow samples of wild-type 129Sv and WASp-deficient mice were processed for electronic microscopy. Different cytologic aspects of MK maturation together with platelet clusters and MK naked nuclei (illustrated in Figure 2) were then scored (219 events) in wild-type 129Sv and WASp-deficient bone marrow EM sections.