List of gene-specific primer pairs used for quantitative real-time PCR
Gene product . | Forward primer . | Reverse primer . |
|---|---|---|
| FIZZ1 | 5′-CCCAGGATGCCAACTTTGAA-3′ | 5′-GGCCCATCTGTTCATAGTCT-3′ |
| Ym | 5′-GGGCATACCTTTATCCTGAG-3′ | 5′-CCACTGAAGTCATCCATGTC-3′ |
| Fibronectin-1 | 5′-CAGCGGATGAGCTGCACATG-3′ | 5′-GTTCTCTGATTGTATCTCTGTG-3′ |
| Arginase-1 | 5′-GCTGTCTTCCCAAGAGTTGGG-3′ | 5′-ATGGAAGAGACCTTCAGCTAC-3′ |
| CCL8 | 5′-TCTACGCAGTGCTTCTTTGC-3′ | 5′-CCACTTCTGTGTGGGGTCTA-3′ |
| CCL24 | 5′-GCTGCACGTCCTTTATTTCC-3′ | 5′-GGCTAAACCTCGGTGCTATT-3′ |
| E-cadherin | 5′-ACTTGGGGACAGCAACATCA-3′ | 5′-GGGTTTAAATCGGCCAGCAT-3′ |
| MGL-1 | 5′-ATGATGTCTGCCAGAGAACC-3′ | 5′-ATCACAGATTTCAGCAACCTTA-3′ |
| MGL-2 | 5′-GATAACTGGCATGGACATATG-3′ | 5′-TTTCTAATCACCATAACACATTC-3′ |
| Selenoprotein P | 5′-TTCTGCAGGCATCCAGATTG-3′ | 5′-CACAAGACGGCCACATCTGT-3′ |
| Coagulation factor XIIIa | 5′-CCAGGAATTAAGCAAGACATC-3′ | 5′-TGCCCTTACTTTCTAGTTCTC-3′ |
| Retinoic acid Rγ | 5′-CTAGTGCCTTCTACCCTTGA-3′ | 5′-AACAGCGGGGTAGGAGATAT-3′ |
| PD-L2 | 5′-GCTTGAGGCGCAGAACACTG-3′ | 5′-TGACCGCAGAGAATGCACTG-3′ |
| TREM-2b | 5′-CTCCGAGGCCGAGAGGC-3′ | 5′-GGGCACCCTCGAAACTCGA-3′ |
| PLA2GIVa | 5′-GAGGGGCTTTATTCCACACG-3′ | 5′-GTCCAAAATCCCCGACTCAT-3′ |
| Cathepsin L | 5′-TGGACTCGGAGGAGTCTTAC-3′ | 5′-TCTGTTCCTTCATAGCCATAG-3′ |
| PLA2GVII/PAF-AH | 5′-TGCACCAGAACTTTGACGAC-3′ | 5′-CAACTGTGTAACAAGCAAGTTC-3′ |
| Macrophage mannose receptor | 5′-CTCGTGGATCTCCGTGACAC-3′ | 5′-GCAAATGGAGCCGTCTGTGC-3′ |
| Prosaposin | 5′-GCCATTGTCATAGCACAGAG-3′ | 5′-CTTCACTCATGAGCTGAACAG-3′ |
| Cysteinyl leukotriene R1 | 5′-GAGCCTCCACAGAACAATCA-3′ | 5′-TAGCAGAGGATCAAAGCAACA-3′ |
| PPARγ | 5′-AGAGTCTGCTGATCTGCGAG-3′ | 5′-GGCATACTCTGTGATCTCTTG-3′ |
| iNOS | 5′-TGGAGCCAAGGCCAAACACAG-3′ | 5′-TCCACCAGGAGATGTTGAAC-3′ |
| 12/15 lipoxygenase | 5′-CAGCTGGATTGGTTCTACTG-3′ | 5′-CTCTGAACTTCTTCAGCACAG-3′ |
| PLA2GIId | 5′-ATTGTTGCTATGCCCACCTG-3′ | 5′-GCACACAGTTGCCTTTCACA-3′ |
| PLA2GV | 5′-GACCGTTGTTATGGGCAACT-3′ | 5′-GAGCCTCATTGGACAGAAGG-3′ |
| PLA2GXIIa | 5′-CCACTGTTTGGCGTTCATCT-3′ | 5′-GGAACTCCTCGTCACAGTCG-3′ |
| PLA2GVIa | 5′-ATGGGAAAGCAGGAGATGGT-3′ | 5′-TCATTTCAGCACACCCCTTC-3′ |
Gene product . | Forward primer . | Reverse primer . |
|---|---|---|
| FIZZ1 | 5′-CCCAGGATGCCAACTTTGAA-3′ | 5′-GGCCCATCTGTTCATAGTCT-3′ |
| Ym | 5′-GGGCATACCTTTATCCTGAG-3′ | 5′-CCACTGAAGTCATCCATGTC-3′ |
| Fibronectin-1 | 5′-CAGCGGATGAGCTGCACATG-3′ | 5′-GTTCTCTGATTGTATCTCTGTG-3′ |
| Arginase-1 | 5′-GCTGTCTTCCCAAGAGTTGGG-3′ | 5′-ATGGAAGAGACCTTCAGCTAC-3′ |
| CCL8 | 5′-TCTACGCAGTGCTTCTTTGC-3′ | 5′-CCACTTCTGTGTGGGGTCTA-3′ |
| CCL24 | 5′-GCTGCACGTCCTTTATTTCC-3′ | 5′-GGCTAAACCTCGGTGCTATT-3′ |
| E-cadherin | 5′-ACTTGGGGACAGCAACATCA-3′ | 5′-GGGTTTAAATCGGCCAGCAT-3′ |
| MGL-1 | 5′-ATGATGTCTGCCAGAGAACC-3′ | 5′-ATCACAGATTTCAGCAACCTTA-3′ |
| MGL-2 | 5′-GATAACTGGCATGGACATATG-3′ | 5′-TTTCTAATCACCATAACACATTC-3′ |
| Selenoprotein P | 5′-TTCTGCAGGCATCCAGATTG-3′ | 5′-CACAAGACGGCCACATCTGT-3′ |
| Coagulation factor XIIIa | 5′-CCAGGAATTAAGCAAGACATC-3′ | 5′-TGCCCTTACTTTCTAGTTCTC-3′ |
| Retinoic acid Rγ | 5′-CTAGTGCCTTCTACCCTTGA-3′ | 5′-AACAGCGGGGTAGGAGATAT-3′ |
| PD-L2 | 5′-GCTTGAGGCGCAGAACACTG-3′ | 5′-TGACCGCAGAGAATGCACTG-3′ |
| TREM-2b | 5′-CTCCGAGGCCGAGAGGC-3′ | 5′-GGGCACCCTCGAAACTCGA-3′ |
| PLA2GIVa | 5′-GAGGGGCTTTATTCCACACG-3′ | 5′-GTCCAAAATCCCCGACTCAT-3′ |
| Cathepsin L | 5′-TGGACTCGGAGGAGTCTTAC-3′ | 5′-TCTGTTCCTTCATAGCCATAG-3′ |
| PLA2GVII/PAF-AH | 5′-TGCACCAGAACTTTGACGAC-3′ | 5′-CAACTGTGTAACAAGCAAGTTC-3′ |
| Macrophage mannose receptor | 5′-CTCGTGGATCTCCGTGACAC-3′ | 5′-GCAAATGGAGCCGTCTGTGC-3′ |
| Prosaposin | 5′-GCCATTGTCATAGCACAGAG-3′ | 5′-CTTCACTCATGAGCTGAACAG-3′ |
| Cysteinyl leukotriene R1 | 5′-GAGCCTCCACAGAACAATCA-3′ | 5′-TAGCAGAGGATCAAAGCAACA-3′ |
| PPARγ | 5′-AGAGTCTGCTGATCTGCGAG-3′ | 5′-GGCATACTCTGTGATCTCTTG-3′ |
| iNOS | 5′-TGGAGCCAAGGCCAAACACAG-3′ | 5′-TCCACCAGGAGATGTTGAAC-3′ |
| 12/15 lipoxygenase | 5′-CAGCTGGATTGGTTCTACTG-3′ | 5′-CTCTGAACTTCTTCAGCACAG-3′ |
| PLA2GIId | 5′-ATTGTTGCTATGCCCACCTG-3′ | 5′-GCACACAGTTGCCTTTCACA-3′ |
| PLA2GV | 5′-GACCGTTGTTATGGGCAACT-3′ | 5′-GAGCCTCATTGGACAGAAGG-3′ |
| PLA2GXIIa | 5′-CCACTGTTTGGCGTTCATCT-3′ | 5′-GGAACTCCTCGTCACAGTCG-3′ |
| PLA2GVIa | 5′-ATGGGAAAGCAGGAGATGGT-3′ | 5′-TCATTTCAGCACACCCCTTC-3′ |