CTL-suppressive macrophages are M2 oriented
Gene product . | Accession no. . | P/N . | Function . |
|---|---|---|---|
| P/N at least 2 | |||
| FIZZ1 | NM_020509 | 438.3 ± 245.9 | Lipid and sugar metabolism |
| Ym | NM_009892 (Ym1); NM_145126 (Ym2) | 357.3 ± 163.3 | Lectin |
| Fibronectin-1 | NM_010233 | 101.3 ± 6.9 | Wound healing |
| Arginase-1 | NM_007482 | 95.6 ± 39.4 | Wound healing, immunomodulation/ROS production |
| CCL8 (MCP-2) | NM_021443 | 17.7 ± 5.2 | Th2-type chemotaxis |
| CCL24 (eotaxin-2) | NM_019577 | 12.0 ± 5.2 | Th2-type chemotaxis |
| E-cadherin | NM_009864 | 11.9 ± 6.6 | Cell-cell adhesion |
| MGL-1 | NM_010796 | 10.3 ± 2.0 | C-type lectin (pattern recognition) |
| MGL-2 | NM_145137 | 9.5 ± 0.8 | C-type lectin (pattern recognition) |
| Selenoprotein P | NM_009155 | 8.5 ± 2.5 | Anti-inflammation |
| Coagulation factor XIIIa | NM_028784 | 5.0 ± 1.2 | Wound healing |
| Retinoic acid Rγ | NM_011244 | 4.7 ± 2.1 | Transcription factor |
| PD-L2 | NM_021396 | 4.6 ± 2.0 | Immunomodulation |
| TREM-2b | AY024349 | 4.6 ± 2.5 | Pattern recognition |
| PLA2GIVa | NM_008869 | 4.1 ± 1.4 | cPLA2; arachidonic acid metabolism |
| Cathepsin L | NM_009984 | 3.7 ± 0.9 | Antigen processing |
| PLA2GVII/PAF-AH | NM_013737 | 3.5 ± 0.8 | Anti-inflammation (arachidonic acid metabolism) |
| Macrophage mannose receptor | NM_008625 | 3.3 ± 1.0 | C-type lectin (pattern recognition) |
| Prosaposin | NM_011179 | 2.9 ± 0.6 | Sphingolipid metabolism |
| Cysteinyl leukotriene R1 | NM_021476 | 2.7 ± 0.6 | Immunomodulation (arachidonic acid metabolism) |
| PPARγ | NM_011146 | 2.4 ± 0.4 | Lipid metabolism, anti-inflammation (arachidonic acid metabolism) |
| P/N less than 2 | |||
| iNOS | NM_010927 | 0.6 ± 0.2 | NO/ROS production |
| 12/15-lipoxygenase | NM_007440 | 0.8 ± 0.3 | Anti-inflammation (arachidonic acid metabolism) |
| PLA2GIId | NM_011109 | 0.9 ± 0.2 | sPLA2, arachidonic acid metabolism |
| PLA2GV | NM_011110 | 1.5 ± 0.5 | sPLA2, arachidonic acid metabolism |
| PLA2GXIIa | NM_023196 | 1.6 ± 0.4 | sPLA2, arachidonic acid metabolism |
| PLA2GVIa | NM_016915 | 2.0 ± 0.5 | iPLA2, arachidonic acid metabolism |
Gene product . | Accession no. . | P/N . | Function . |
|---|---|---|---|
| P/N at least 2 | |||
| FIZZ1 | NM_020509 | 438.3 ± 245.9 | Lipid and sugar metabolism |
| Ym | NM_009892 (Ym1); NM_145126 (Ym2) | 357.3 ± 163.3 | Lectin |
| Fibronectin-1 | NM_010233 | 101.3 ± 6.9 | Wound healing |
| Arginase-1 | NM_007482 | 95.6 ± 39.4 | Wound healing, immunomodulation/ROS production |
| CCL8 (MCP-2) | NM_021443 | 17.7 ± 5.2 | Th2-type chemotaxis |
| CCL24 (eotaxin-2) | NM_019577 | 12.0 ± 5.2 | Th2-type chemotaxis |
| E-cadherin | NM_009864 | 11.9 ± 6.6 | Cell-cell adhesion |
| MGL-1 | NM_010796 | 10.3 ± 2.0 | C-type lectin (pattern recognition) |
| MGL-2 | NM_145137 | 9.5 ± 0.8 | C-type lectin (pattern recognition) |
| Selenoprotein P | NM_009155 | 8.5 ± 2.5 | Anti-inflammation |
| Coagulation factor XIIIa | NM_028784 | 5.0 ± 1.2 | Wound healing |
| Retinoic acid Rγ | NM_011244 | 4.7 ± 2.1 | Transcription factor |
| PD-L2 | NM_021396 | 4.6 ± 2.0 | Immunomodulation |
| TREM-2b | AY024349 | 4.6 ± 2.5 | Pattern recognition |
| PLA2GIVa | NM_008869 | 4.1 ± 1.4 | cPLA2; arachidonic acid metabolism |
| Cathepsin L | NM_009984 | 3.7 ± 0.9 | Antigen processing |
| PLA2GVII/PAF-AH | NM_013737 | 3.5 ± 0.8 | Anti-inflammation (arachidonic acid metabolism) |
| Macrophage mannose receptor | NM_008625 | 3.3 ± 1.0 | C-type lectin (pattern recognition) |
| Prosaposin | NM_011179 | 2.9 ± 0.6 | Sphingolipid metabolism |
| Cysteinyl leukotriene R1 | NM_021476 | 2.7 ± 0.6 | Immunomodulation (arachidonic acid metabolism) |
| PPARγ | NM_011146 | 2.4 ± 0.4 | Lipid metabolism, anti-inflammation (arachidonic acid metabolism) |
| P/N less than 2 | |||
| iNOS | NM_010927 | 0.6 ± 0.2 | NO/ROS production |
| 12/15-lipoxygenase | NM_007440 | 0.8 ± 0.3 | Anti-inflammation (arachidonic acid metabolism) |
| PLA2GIId | NM_011109 | 0.9 ± 0.2 | sPLA2, arachidonic acid metabolism |
| PLA2GV | NM_011110 | 1.5 ± 0.5 | sPLA2, arachidonic acid metabolism |
| PLA2GXIIa | NM_023196 | 1.6 ± 0.4 | sPLA2, arachidonic acid metabolism |
| PLA2GVIa | NM_016915 | 2.0 ± 0.5 | iPLA2, arachidonic acid metabolism |
Splenocytes from 3 individual BW-Sp3 progressors and 2 individual naive mice were restimulated in vitro with irradiated BW-Sp3(B7-1) for 5 days, after which RNA was extracted and cDNA prepared from the adherent splenocytes. Expression of each of the listed genes was determined via quantitative real-time PCR and normalized for the housekeeping gene ribosomal protein S12. The fold induction of gene expression (mean ± SD of 3 individual mice) in progressor compared with naive adherent splenocytes (mean ± SD of 2 individual mice) is shown (P/N).
P/N at least 2 indicates all tested genes with a minimal 2-fold induction in progressor adherent splenocytes, a criterion that is not met by the genes listed under P/N less than 2.