Real-time PCR primers
Target . | Sense primer . | Position on cDNA . | Antisense primer . | Position on cDNA . | Probe . | Position on cDNA . |
|---|---|---|---|---|---|---|
| BKB1R | 5′-CACTTTGCAAGGATGGTGGAGTTG-3′ | 764-787 | 5′-GGAGGCCAGGATGTGATAGTTGAA-3′ | 830-853 | —* | — |
| BKB2R | 5′-CTGGGTGTTTGGAGAGGTGT-3′ | 479-499 | 5′-ACGAGCATCAGGAAGCAGAT-3′ | 565-545 | 56-FAM/GGGTGGTGAACACCATGATC/3BHQ-1 | 505-525 |
| AT1R | 5′-CAGTGGAGAAGAGCCCAGTC-3′ | 679-699 | 5′-AGCACTCTGGGCAGTTCAGT-3′ | 821-801 | 56-FAM/TTCCCCTCCTACCCCATAAC/3BHQ-1 | 768-788 |
| AT2R | 5′-CCCTAAAAAGGTGTCCAGCA-3′ | 395-415 | 5′-CACAGGTCCAAAAAGCCAAT-3′ | 518-498 | 56-FAM/ATCTGGCCTTGGCTGACTTA/3BHQ-1 | 427-447 |
| MAS | 5′-TCTACTTGGGGATCGACTGG-3′ | 909-928 | 5′-GCACTGCTGTTGATGCAGAT-3′ | 1008-989 | 56-FAM/CCCCGAGTACGTCACTGACT/3BHQ-1 | 964-983 |
| PK | 5′-GGCTACACGAAGGAACAAGG3′ | 1588-1607 | 5′-CACCGGAATCTCCCTTACAA-3′ | 1759-1740 | 56-FAM/GTGCTGGCTACAAAGAAGGG/3BHQ-1 | 1709-1727 |
| FXI | 5′-GCAAATACAATGGGGTCTGGC-3 | 1809-1829 | 5′-CCAGTTGAACTCTTTCAGACTGTTT3′ | 1988-1968 | 56-FAM/GCCAAGTACGTGGACTGGAT/3BHQ-1 | 1901-1920 |
| PAI-1 | 5′-AGTCTTTCCGACCAAGAGCA-3′ | 1122-1142 | 5′-GCCGAACCACAAAGAGAAAG-3′ | 1330-1310 | 56-FAM/GATCGAGGTAAACGAGAGCG/3BHQ-1 | 1186-1205 |
| γ-actin | 5′-CGAAAAGACCTGTATGCCAAT-3 | 910-927 | 5′-GGGCTGTGATCTCCTTCTGC-3′ | 997-978 | HEX/TACCACCATGTACCCAGGCATTGCTGA/3BHQ-1 | 945-971 |
Target . | Sense primer . | Position on cDNA . | Antisense primer . | Position on cDNA . | Probe . | Position on cDNA . |
|---|---|---|---|---|---|---|
| BKB1R | 5′-CACTTTGCAAGGATGGTGGAGTTG-3′ | 764-787 | 5′-GGAGGCCAGGATGTGATAGTTGAA-3′ | 830-853 | —* | — |
| BKB2R | 5′-CTGGGTGTTTGGAGAGGTGT-3′ | 479-499 | 5′-ACGAGCATCAGGAAGCAGAT-3′ | 565-545 | 56-FAM/GGGTGGTGAACACCATGATC/3BHQ-1 | 505-525 |
| AT1R | 5′-CAGTGGAGAAGAGCCCAGTC-3′ | 679-699 | 5′-AGCACTCTGGGCAGTTCAGT-3′ | 821-801 | 56-FAM/TTCCCCTCCTACCCCATAAC/3BHQ-1 | 768-788 |
| AT2R | 5′-CCCTAAAAAGGTGTCCAGCA-3′ | 395-415 | 5′-CACAGGTCCAAAAAGCCAAT-3′ | 518-498 | 56-FAM/ATCTGGCCTTGGCTGACTTA/3BHQ-1 | 427-447 |
| MAS | 5′-TCTACTTGGGGATCGACTGG-3′ | 909-928 | 5′-GCACTGCTGTTGATGCAGAT-3′ | 1008-989 | 56-FAM/CCCCGAGTACGTCACTGACT/3BHQ-1 | 964-983 |
| PK | 5′-GGCTACACGAAGGAACAAGG3′ | 1588-1607 | 5′-CACCGGAATCTCCCTTACAA-3′ | 1759-1740 | 56-FAM/GTGCTGGCTACAAAGAAGGG/3BHQ-1 | 1709-1727 |
| FXI | 5′-GCAAATACAATGGGGTCTGGC-3 | 1809-1829 | 5′-CCAGTTGAACTCTTTCAGACTGTTT3′ | 1988-1968 | 56-FAM/GCCAAGTACGTGGACTGGAT/3BHQ-1 | 1901-1920 |
| PAI-1 | 5′-AGTCTTTCCGACCAAGAGCA-3′ | 1122-1142 | 5′-GCCGAACCACAAAGAGAAAG-3′ | 1330-1310 | 56-FAM/GATCGAGGTAAACGAGAGCG/3BHQ-1 | 1186-1205 |
| γ-actin | 5′-CGAAAAGACCTGTATGCCAAT-3 | 910-927 | 5′-GGGCTGTGATCTCCTTCTGC-3′ | 997-978 | HEX/TACCACCATGTACCCAGGCATTGCTGA/3BHQ-1 | 945-971 |
BKB1R indicates bradykinin B1 receptor; BKB2R, bradykinin B2 receptor; AT1R, angiotensin receptor type 1; AT2R, angiotensin receptor type 2; MAS, mas protooncogene or the angiotensin 1-7 receptor; PK, prekallikrein; FXI, factor XI; PAI-1, plasminogen activator inhibitor-1.
Detection of the PCR product was with SYBR-490. See “Materials and methods.”