Real-time PCR receptor mRNA expression in BKB2R−/− mice
. | Ratio of mean fold-change in gene expression* . | . | . | ||
|---|---|---|---|---|---|
| Receptor . | Heart . | Kidney . | Liver . | ||
| BKB1R | 1.9 | 1.7 | — | ||
| AT1R | 0.06 | 1.0 | 1.0 | ||
| AT2R | 8.0 | 16.0 | 4.0 | ||
| MAS | 0.93 | 0.57 | 1.62 | ||
. | Ratio of mean fold-change in gene expression* . | . | . | ||
|---|---|---|---|---|---|
| Receptor . | Heart . | Kidney . | Liver . | ||
| BKB1R | 1.9 | 1.7 | — | ||
| AT1R | 0.06 | 1.0 | 1.0 | ||
| AT2R | 8.0 | 16.0 | 4.0 | ||
| MAS | 0.93 | 0.57 | 1.62 | ||
Values for the Ct for each real-time PCR for each receptor were used to calculate the fold-change of the cDNA for the target gene compared with that of γ-actin by the formula 2−ΔΔCt, where ΔΔCt − (CtTarget − CtActin) Time X − (CtTarget − CtActin) Time 0 (see “Materials and methods”).29 Final values in the table are expressed as the ratio of the fold-change in the target gene in the knockout mouse over the fold-change in the target gene of the control mouse. These data are means of 3 to 5 individual experiments.
— indicates that the assay was not done.