rKSHV.219 infection in NOD/SCID-hu mice
. | . | . | KSHV PCRd . | . | . | CD14+/GFP+e, % . | . | CD19+/GFP+f, % . | . | ||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Groupa, mouse ID . | No. CD34+ cellsb, × 107 . | Weeksc . | Spleen . | Bone marrow . | Peripheral blood . | Spleen . | Bone marrow . | Spleen . | Bone marrow . | ||||
| MOCK | |||||||||||||
| D8g | 0.1 | 8 | − | − | − | 0 | 0 | 0 | 0 | ||||
| D9g | 0.1 | 20 | − | − | − | 0 | 0 | 0 | 0 | ||||
| D10g | 0.5 | 8 | − | − | − | 0 | 0 | 0 | 0 | ||||
| D11gh | 0.5 | 7 | nd | nd | − | nd | nd | nd | nd | ||||
| D51g | 0.5 | 17 | − | − | − | 0 | 0 | 0 | 0 | ||||
| D52g | 0.5 | 3 | − | − | nd | 0 | 0 | 0 | 0 | ||||
| D55g | 0.5 | 17 | − | − | − | 0 | 0 | 0 | 0 | ||||
| D57g | 0.5 | 4 | − | − | nd | 0 | 0 | 0 | 0 | ||||
| D59g | 0.5 | 5 | − | − | nd | 0 | 0 | 0 | 0 | ||||
| D13g | 1.0 | 12 | − | − | − | 0 | 0 | 0 | 0 | ||||
| D1 | 2.0 | 8 | − | − | nd | 0 | 0 | 0 | 0 | ||||
| D2 | 2.0 | 10 | − | − | nd | 0 | 0 | 0 | 0 | ||||
| HEAT | |||||||||||||
| D3 | 2.0 | 8 | − | − | nd | 0 | 0 | 0 | 0 | ||||
| D4 | 2.0 | 10 | − | − | nd | 0 | 0 | 0 | 0 | ||||
| rKSHV.219 | |||||||||||||
| D14gi | 0.1 | 3 | +++ | +++ | nd | nd | nd | nd | nd | ||||
| D15g | 0.1 | 8 | + | + | + | 0 | 0 | 0 | 0 | ||||
| D16g | 0.1 | 29 | + | + | + | 3.6 | 0.6 | 2.5 | 0.6 | ||||
| D17gj | 0.1 | 19 | ++ | +++ | +++ | 0.5 | 0.9 | 0.3 | 0.9 | ||||
| D18gk | 0.5 | 8 | +++ | ++ | + | 3.8 | 0.8 | 0 | 0 | ||||
| D19gj | 0.5 | 14 | ++ | ++ | + | 1.4 | 0.3 | 0 | 0 | ||||
| D20g | 0.5 | 14 | − | − | − | 0 | 0 | 0 | 0 | ||||
| D21gj | 0.5 | 23 | + | + | + | 2.7 | 2.7 | 2.3 | 2.7 | ||||
| D67g | 0.5 | 17 | + | + | + | 8.9 | 3.4 | 2.8 | 1.2 | ||||
| D73g | 0.5 | 17 | + | + | + | 9.7 | 1.7 | 2.7 | 0.5 | ||||
| D22g | 1.0 | 8 | + | ++ | + | 0 | 0 | 0 | 0 | ||||
| D23g | 1.0 | 14 | + | + | ++ | 0 | 0 | 0 | 0 | ||||
| D25g | 1.0 | 13 | − | − | − | 0 | 0 | 0 | 0 | ||||
| D5 | 2.0 | 8 | ++ | ++ | nd | 2.7 | 4.3 | 4.5 | 6.4 | ||||
| D6 | 2.0 | 10 | ++ | +++ | nd | 4.5 | 2.6 | 0.5 | 2.0 | ||||
| D7 | 2.0 | 10 | +++ | ++ | nd | 5.9 | 0 | 0.4 | 0 | ||||
. | . | . | KSHV PCRd . | . | . | CD14+/GFP+e, % . | . | CD19+/GFP+f, % . | . | ||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Groupa, mouse ID . | No. CD34+ cellsb, × 107 . | Weeksc . | Spleen . | Bone marrow . | Peripheral blood . | Spleen . | Bone marrow . | Spleen . | Bone marrow . | ||||
| MOCK | |||||||||||||
| D8g | 0.1 | 8 | − | − | − | 0 | 0 | 0 | 0 | ||||
| D9g | 0.1 | 20 | − | − | − | 0 | 0 | 0 | 0 | ||||
| D10g | 0.5 | 8 | − | − | − | 0 | 0 | 0 | 0 | ||||
| D11gh | 0.5 | 7 | nd | nd | − | nd | nd | nd | nd | ||||
| D51g | 0.5 | 17 | − | − | − | 0 | 0 | 0 | 0 | ||||
| D52g | 0.5 | 3 | − | − | nd | 0 | 0 | 0 | 0 | ||||
| D55g | 0.5 | 17 | − | − | − | 0 | 0 | 0 | 0 | ||||
| D57g | 0.5 | 4 | − | − | nd | 0 | 0 | 0 | 0 | ||||
| D59g | 0.5 | 5 | − | − | nd | 0 | 0 | 0 | 0 | ||||
| D13g | 1.0 | 12 | − | − | − | 0 | 0 | 0 | 0 | ||||
| D1 | 2.0 | 8 | − | − | nd | 0 | 0 | 0 | 0 | ||||
| D2 | 2.0 | 10 | − | − | nd | 0 | 0 | 0 | 0 | ||||
| HEAT | |||||||||||||
| D3 | 2.0 | 8 | − | − | nd | 0 | 0 | 0 | 0 | ||||
| D4 | 2.0 | 10 | − | − | nd | 0 | 0 | 0 | 0 | ||||
| rKSHV.219 | |||||||||||||
| D14gi | 0.1 | 3 | +++ | +++ | nd | nd | nd | nd | nd | ||||
| D15g | 0.1 | 8 | + | + | + | 0 | 0 | 0 | 0 | ||||
| D16g | 0.1 | 29 | + | + | + | 3.6 | 0.6 | 2.5 | 0.6 | ||||
| D17gj | 0.1 | 19 | ++ | +++ | +++ | 0.5 | 0.9 | 0.3 | 0.9 | ||||
| D18gk | 0.5 | 8 | +++ | ++ | + | 3.8 | 0.8 | 0 | 0 | ||||
| D19gj | 0.5 | 14 | ++ | ++ | + | 1.4 | 0.3 | 0 | 0 | ||||
| D20g | 0.5 | 14 | − | − | − | 0 | 0 | 0 | 0 | ||||
| D21gj | 0.5 | 23 | + | + | + | 2.7 | 2.7 | 2.3 | 2.7 | ||||
| D67g | 0.5 | 17 | + | + | + | 8.9 | 3.4 | 2.8 | 1.2 | ||||
| D73g | 0.5 | 17 | + | + | + | 9.7 | 1.7 | 2.7 | 0.5 | ||||
| D22g | 1.0 | 8 | + | ++ | + | 0 | 0 | 0 | 0 | ||||
| D23g | 1.0 | 14 | + | + | ++ | 0 | 0 | 0 | 0 | ||||
| D25g | 1.0 | 13 | − | − | − | 0 | 0 | 0 | 0 | ||||
| D5 | 2.0 | 8 | ++ | ++ | nd | 2.7 | 4.3 | 4.5 | 6.4 | ||||
| D6 | 2.0 | 10 | ++ | +++ | nd | 4.5 | 2.6 | 0.5 | 2.0 | ||||
| D7 | 2.0 | 10 | +++ | ++ | nd | 5.9 | 0 | 0.4 | 0 | ||||
Real-time PCR and flow cytometric analysis of spleen, bone marrow, and peripheral blood samples recovered from NOD/SCID mice intravenously inoculated with CD34+ HPCs infected with rKSHV.219.
nd indicates not done.
CD34+ HPCs were infected with rKSHV.219, heat-treated rKSHV.219 (HEAT), or mock-infected (MOCK).
Number of CD34+ HPCs inoculated intravenously via tail vein injection.
Weeks after inoculation at which mouse was killed.
Real-time PCR analysis of DNA extracted from indicated tissues using primers specific for KSHV (KS330233). KSHV PCR signal was standardized to the number of human cells detected as determined by human β-globin gene signal. Calculated KSHV genome equivalents per human cell: −, (0); +, (<0.1); ++, (0.1-1.0); +++, (>1.0).
Flow cytometric analysis of cells recovered from indicated tissues using antibodies directed against human CD14. Numbers indicate percentage of gated cells that co-express human CD14 and GFP.
Flow cytometric analysis of cells recovered from indicated tissues using antibodies directed against human CD19. Numbers indicate percentage of gated cells that co-express human CD19 and GFP.
Mouse sublethally irradiated (300 rad) 2 days prior to tail vein inoculation.
Peripheral blood sample collected prior to death. Tissues not available due to decomposition.
Sufficient spleen and bone marrow samples for PCR analysis only.
Pleural effusion. Fluid (400-1000 μl) was recovered from pleural cavity of mouse and contained transformed murine B cells.
Ocular inoculation of rKSHV.219-infected CD34+ HPCs.