Analysis of cell lines from patients
Patient . | d* . | No. of GPI- cells . | No. of GPI+ cells . | f,†× 106 . | μ,‡× 107 . |
|---|---|---|---|---|---|
| 1 | 4.24 | 4 | 846 083 | 4.73 | 11.2 |
| 2 | 10.6 | 4 | 792 311 | 5.05 | 4.76 |
| 3 | 9.5 | 1 | 849 779 | 1.18 | 1.24 |
| 4 | 6.49 | 4 | 2 934 988 | 1.36 | 2.10 |
| 5 | 6.07 | 5 | 2 342 270 | 2.13 | 3.51 |
Patient . | d* . | No. of GPI- cells . | No. of GPI+ cells . | f,†× 106 . | μ,‡× 107 . |
|---|---|---|---|---|---|
| 1 | 4.24 | 4 | 846 083 | 4.73 | 11.2 |
| 2 | 10.6 | 4 | 792 311 | 5.05 | 4.76 |
| 3 | 9.5 | 1 | 849 779 | 1.18 | 1.24 |
| 4 | 6.49 | 4 | 2 934 988 | 1.36 | 2.10 |
| 5 | 6.07 | 5 | 2 342 270 | 2.13 | 3.51 |
Number of cell divisions occurring in culture after sorting. In cell lines from the 5 patients, we collected by flow sorting a mean of 2 × 106 live cells, which proliferated in culture for a mean of 32 days, undergoing a mean of 7.4 cell divisions.
Frequency (per 106) of GPI- cells after expansion after sorting: f = number of GPI- cells ÷ number of GPI+ cells.
Mutation rate (mutations per 107 cell divisions): μ = f ÷ d.