Competitive reconstitution of irradiated mice with various fetal liver HSC populations reveals that CD48 enhances the purity of fetal liver HSCs
Donor cells . | Cell dose . | Mice with engrafted cells . | Frequency of engrafted cells . | Mice with long-term multilineage reconstitution . | Frequency of mice that were long-term multilineage reconstituted (%) . |
|---|---|---|---|---|---|
| TSLM | 3 | 4 of 10 | 1 in 6.4 | 3 of 10 | 1 in 8.9 (11) |
| CD48- SLM | 10 | 3 of 4 | 1 in 7.7 | 3 of 4 | 1 in 7.7 (13) |
| CD48+ SLM | 10 | 0 of 8 | NA | NA | NA |
| CD48- TSLM | 3 | 15 of 21 | 1 in 2.9 | 10 of 21 | 1 in 5.2 (19) |
| CD48- TSLM | 1 | 9 of 14 | 1 in 1.6 | 5 of 14 | 1 in 2.8 (36) |
Donor cells . | Cell dose . | Mice with engrafted cells . | Frequency of engrafted cells . | Mice with long-term multilineage reconstitution . | Frequency of mice that were long-term multilineage reconstituted (%) . |
|---|---|---|---|---|---|
| TSLM | 3 | 4 of 10 | 1 in 6.4 | 3 of 10 | 1 in 8.9 (11) |
| CD48- SLM | 10 | 3 of 4 | 1 in 7.7 | 3 of 4 | 1 in 7.7 (13) |
| CD48+ SLM | 10 | 0 of 8 | NA | NA | NA |
| CD48- TSLM | 3 | 15 of 21 | 1 in 2.9 | 10 of 21 | 1 in 5.2 (19) |
| CD48- TSLM | 1 | 9 of 14 | 1 in 1.6 | 5 of 14 | 1 in 2.8 (36) |
The indicated number of donor-type (CD45.2+) cells from fetal liver were transplanted intravenously into lethally irradiated adult recipients (CD45.1+) along with 200 000 recipient-type whole bone marrow cells for radioprotection. Recipients were considered engrafted by donor cells if any CD45.2+ cells were detected in their peripheral blood (above background: > 0.3% of white blood cells). Mice were considered long-term multilineage reconstituted if donor-type myeloid, B, and T cells were present for more than 16 weeks after reconstitution. The frequency of HSCs was calculated by Poisson limit-dilution statistics6,26 based on the frequency of mice that became long-term multilineage reconstituted.
TSLM indicates ThylowSca-1+lineage-Mac-1+ fetal liver cells; NA, not applicable.