Competitive reconstitution of irradiated mice with various fetal liver HSC populations reveals that CD150 enhances the purity of fetal liver HSCs
Donor cells . | Cell dose . | Mice with engrafted cells . | Frequency of engrafted cells . | Mice with long-term multilineage reconstitution . | Frequency of mice that were long-term multilineage reconstituted (%) . |
|---|---|---|---|---|---|
| CD150-SLM | 10 | 6 of 7 | 1 in 5.7 | 1 of 7 | 1 in 65.4 (1.5) |
| CD150+SLM | 10 | 6 of 7 | 1 in 5.7 | 4 of 7 | 1 in 12.3 (8) |
| CD150+CD48-SLM | 1 | 9 of 17 | 1 in 1.9 | 7 of 17 | 1 in 2.4 (42) |
| CD150+CD48-SLM | 1 | 5 of 13 | 1 in 2.6 | 4 of 13 | 1 in 3.3 (30) |
| CD150+CD48-CD41- | 1 | 3 of 17 | 1 in 5.7 | 3 of 17 | 1 in 5.7 (18) |
| CD150+CD48-CD41- | 1 | 6 of 23 | 1 in 3.8 | 4 of 23 | 1 in 5.8 (17) |
Donor cells . | Cell dose . | Mice with engrafted cells . | Frequency of engrafted cells . | Mice with long-term multilineage reconstitution . | Frequency of mice that were long-term multilineage reconstituted (%) . |
|---|---|---|---|---|---|
| CD150-SLM | 10 | 6 of 7 | 1 in 5.7 | 1 of 7 | 1 in 65.4 (1.5) |
| CD150+SLM | 10 | 6 of 7 | 1 in 5.7 | 4 of 7 | 1 in 12.3 (8) |
| CD150+CD48-SLM | 1 | 9 of 17 | 1 in 1.9 | 7 of 17 | 1 in 2.4 (42) |
| CD150+CD48-SLM | 1 | 5 of 13 | 1 in 2.6 | 4 of 13 | 1 in 3.3 (30) |
| CD150+CD48-CD41- | 1 | 3 of 17 | 1 in 5.7 | 3 of 17 | 1 in 5.7 (18) |
| CD150+CD48-CD41- | 1 | 6 of 23 | 1 in 3.8 | 4 of 23 | 1 in 5.8 (17) |
The indicated number of donor-type (CD45.2+) cells from fetal liver were transplanted intravenously into lethally irradiated recipients (CD45.1+) along with recipient-type 200 000 whole bone marrow cells for radioprotection. Recipients were considered engrafted by donor cells if any CD45.2+ cells were detected in their peripheral blood (above background: > 0.3% of white blood cells). Mice were considered long-term multilineage reconstituted if donor-type myeloid, B, and T cells were present for more than 16 weeks after reconstitution. The frequency of HSCs was calculated by Poisson limit-dilution statistics6,26 based on the frequency of mice that became long-term multilineage reconstituted.