Macrophage protection by ACM
ACM treatment . | Protection, %* . | SD . | P, n ≥ 3† . |
|---|---|---|---|
| None | 100 | ± 0 | ND |
| Heat inactivation, 100°C | 87.4 | ± 2.4 | .4 |
| Proteinase K digestion, 50 μM | 98.5 | ± 8.2 | .39 |
| Trypsin digestion, 0.1% | 99.2 | ± 12 | .46 |
| Chloroform extraction | –5.2 | ± 17.5 | .007 |
ACM treatment . | Protection, %* . | SD . | P, n ≥ 3† . |
|---|---|---|---|
| None | 100 | ± 0 | ND |
| Heat inactivation, 100°C | 87.4 | ± 2.4 | .4 |
| Proteinase K digestion, 50 μM | 98.5 | ± 8.2 | .39 |
| Trypsin digestion, 0.1% | 99.2 | ± 12 | .46 |
| Chloroform extraction | –5.2 | ± 17.5 | .007 |
ND indicates not determined
100% protection is set as the difference in caspase-3 activity (fold of control) between untreated and ACM-treated (16 hours) THP-1 macrophages after induction of apoptosis with 250 μM etoposide for 8 hours. Caspase-3 activity in whole-cell lysates was quantified as described in “Materials and methods”
Statistical analysis was performed using the paired Student t test