Phenotypic and functional changes in lymphocyte subsets after about 4 weeks of stimulation with or without CMV loading
. | Control culture, mean % ± SD . | . | CMV-pulsed culture, mean % ± SD . | . | ||
|---|---|---|---|---|---|---|
| Lymphocyte subsets . | Start . | End . | Start . | End (no. of experiments) . | ||
| CD3+ | 77 ± 11 | 98 ± 2 | 77 ± 12 | 99 ± 0.5 (5) | ||
| CD4+/CD3+ | 78 ± 10 | 72 ± 4 | 78 ± 10 | 78 ± 25 (5) | ||
| CD8+/CD3+ | 28 ± 13 | 37 ± 5 | 28 ± 13 | 27 ± 27 (5) | ||
| CD45RA+/RO– | 55 ± 32 | 55 ± 33 | 50 ± 35 | 16 ± 6 (4) | ||
| CD45RA–/RO+ | 33 ± 32 | 35 ± 29 | 40 ± 39 | 73 ± 13 (4) | ||
| CD45RA+/CD4+ | 58 ± 28 | 57 ± 30 | 51 ± 9 | 18 ± 5 (3) | ||
| CD45RA+/CD8+ | 65 ± 24 | 71 ± 27 | 67 ± 19 | 35 ± 25 (3) | ||
| CD45RA+/CD62L+ | 56 ± 27 | 58 ± 30 | 49 ± 33 | 16 ± 6 (3) | ||
| CD45RA+/CD27+/CD8+ | 65 ± 32 | 68 ± 31 | 69 ± 25 | 30 ± 27 (3) | ||
| CD45RA–/CD27+/CD8+ | 31 ± 30 | 28 ± 31 | 26 ± 23 | 56 ± 26 (3) | ||
| IL-2+/CD3+ T cells | ND | 0 ± 0 | ND | 14 ± 16 (4) | ||
| IFN-γ+/CD3+ T cells | ND | 1 ± 1 | ND | 26 ± 17 (5) | ||
| IFN-γ+/CD4+ T cells | ND | 1 ± 1 | ND | 29 ± 17 (5) | ||
| IFN-γ+/CD8+ T cells | ND | 1 ± 1 | ND | 17 ± 12 (5) | ||
| TNF-α+/CD3+ T cells | ND | 2 ± 2 | ND | 33 ± 21 (5) | ||
| TNF-α+/CD4+ T cells | ND | 2 ± 2 | ND | 39 ± 20 (5) | ||
| TNF-α+/CD8+ T cells | ND | 1 ± 2 | ND | 15 ± 15 (5) | ||
. | Control culture, mean % ± SD . | . | CMV-pulsed culture, mean % ± SD . | . | ||
|---|---|---|---|---|---|---|
| Lymphocyte subsets . | Start . | End . | Start . | End (no. of experiments) . | ||
| CD3+ | 77 ± 11 | 98 ± 2 | 77 ± 12 | 99 ± 0.5 (5) | ||
| CD4+/CD3+ | 78 ± 10 | 72 ± 4 | 78 ± 10 | 78 ± 25 (5) | ||
| CD8+/CD3+ | 28 ± 13 | 37 ± 5 | 28 ± 13 | 27 ± 27 (5) | ||
| CD45RA+/RO– | 55 ± 32 | 55 ± 33 | 50 ± 35 | 16 ± 6 (4) | ||
| CD45RA–/RO+ | 33 ± 32 | 35 ± 29 | 40 ± 39 | 73 ± 13 (4) | ||
| CD45RA+/CD4+ | 58 ± 28 | 57 ± 30 | 51 ± 9 | 18 ± 5 (3) | ||
| CD45RA+/CD8+ | 65 ± 24 | 71 ± 27 | 67 ± 19 | 35 ± 25 (3) | ||
| CD45RA+/CD62L+ | 56 ± 27 | 58 ± 30 | 49 ± 33 | 16 ± 6 (3) | ||
| CD45RA+/CD27+/CD8+ | 65 ± 32 | 68 ± 31 | 69 ± 25 | 30 ± 27 (3) | ||
| CD45RA–/CD27+/CD8+ | 31 ± 30 | 28 ± 31 | 26 ± 23 | 56 ± 26 (3) | ||
| IL-2+/CD3+ T cells | ND | 0 ± 0 | ND | 14 ± 16 (4) | ||
| IFN-γ+/CD3+ T cells | ND | 1 ± 1 | ND | 26 ± 17 (5) | ||
| IFN-γ+/CD4+ T cells | ND | 1 ± 1 | ND | 29 ± 17 (5) | ||
| IFN-γ+/CD8+ T cells | ND | 1 ± 1 | ND | 17 ± 12 (5) | ||
| TNF-α+/CD3+ T cells | ND | 2 ± 2 | ND | 33 ± 21 (5) | ||
| TNF-α+/CD4+ T cells | ND | 2 ± 2 | ND | 39 ± 20 (5) | ||
| TNF-α+/CD8+ T cells | ND | 1 ± 2 | ND | 15 ± 15 (5) | ||
ND indicates not determined