Composition of the cell populations and expression of CD69, BLT1, CXCR4, CXCR3, and CCR5 in ex vivo CBMCs and 6-day-old cultures
| Cells . | Ex vivo . | CBMCs . | CBMCs + EBV . | CBMCs + EBV + PSK . | CBMCs + EBV + Trx80 . |
|---|---|---|---|---|---|
| CD3 T | 66 ± 9 | 59 ± 11 | 44 ± 7 | 61 ± 6 | 59 ± 6 |
| CD56 NK | 12 ± 7 | 13 ± 5 | 15 ± 2 | 19 ± 4 | 13 ± 3 |
| CD19 B | 14 ± 5 | 15 ± 4 | 41 ± 6 | 19 ± 7 | 22 ± 4 |
| CD69+CD3+/CD3 | 3 ± 2 | 8 ± 4 | 26 ± 5 | 71 ± 18 | 68 ± 16 |
| CD69+CD56+/CD56 | 8 ± 2 | 16 ± 3 | 44 ± 10 | 69 ± 8 | 71 ± 14 |
| BLT1+CD3+/CD3 | 0 | 1 | 33 ± 8 | 37 ± 6 | 41 ± 10 |
| BLT1+CD56+/CD56 | 0 | 0 | 0 | 0 | 0 |
| BLT1+CD19/CD19 | 51 ± 9 | 11 ± 4 | 7 ± 2 | 8 ± 6 | 9 ± 4 |
| CXCR4+CD3+/CD3 | 86 ± 6 | 16 ± 9 | 5 ± 2 | 3 ± 1 | 3 ± 3 |
| CXCR3+CD3+/CD3 | 3 ± 1 | 2 ± 2 | 51 ± 11 | 57 ± 14 | 56 ± 6 |
| CCR5+CD3+/CD3 | 4 ± 3 | 3 ± 2 | 54 ± 5 | 56 ± 7 | 61 ± 11 |
| Cells . | Ex vivo . | CBMCs . | CBMCs + EBV . | CBMCs + EBV + PSK . | CBMCs + EBV + Trx80 . |
|---|---|---|---|---|---|
| CD3 T | 66 ± 9 | 59 ± 11 | 44 ± 7 | 61 ± 6 | 59 ± 6 |
| CD56 NK | 12 ± 7 | 13 ± 5 | 15 ± 2 | 19 ± 4 | 13 ± 3 |
| CD19 B | 14 ± 5 | 15 ± 4 | 41 ± 6 | 19 ± 7 | 22 ± 4 |
| CD69+CD3+/CD3 | 3 ± 2 | 8 ± 4 | 26 ± 5 | 71 ± 18 | 68 ± 16 |
| CD69+CD56+/CD56 | 8 ± 2 | 16 ± 3 | 44 ± 10 | 69 ± 8 | 71 ± 14 |
| BLT1+CD3+/CD3 | 0 | 1 | 33 ± 8 | 37 ± 6 | 41 ± 10 |
| BLT1+CD56+/CD56 | 0 | 0 | 0 | 0 | 0 |
| BLT1+CD19/CD19 | 51 ± 9 | 11 ± 4 | 7 ± 2 | 8 ± 6 | 9 ± 4 |
| CXCR4+CD3+/CD3 | 86 ± 6 | 16 ± 9 | 5 ± 2 | 3 ± 1 | 3 ± 3 |
| CXCR3+CD3+/CD3 | 3 ± 1 | 2 ± 2 | 51 ± 11 | 57 ± 14 | 56 ± 6 |
| CCR5+CD3+/CD3 | 4 ± 3 | 3 ± 2 | 54 ± 5 | 56 ± 7 | 61 ± 11 |
Uninfected CBMCs, EBV-infected CBMCs without and with PSK (25 μg/mL), and EBV-infected CBMCs with Trx80 (100 nM) were cultured for 6 days. Expression of CD3, CD56, CD19, CD69, BLT1, CXCR4, CXCR3, and CCR5 was registered by flow cytometry and double staining. Results represent means plus or minus SD of 4 experiments.