Composition of the cell populations in 20-day-old cultures initiated with EBV-infected CBMCs (%)
| Cells . | CBMCs plus EBV plus LTB4* . | CBMCs plus EBV . |
|---|---|---|
| CD19 B | 11 ± 6 | 89 ± 7 |
| CD4 T | 71 ± 7 | 3 ± 3 |
| CD8 T | 6 ± 5 | 2 ± 1 |
| CD56 NK | 6 ± 3 | 4 ± 2 |
| CD3 T | 79 ± 8 | 5 ± 3 |
| Cells . | CBMCs plus EBV plus LTB4* . | CBMCs plus EBV . |
|---|---|---|
| CD19 B | 11 ± 6 | 89 ± 7 |
| CD4 T | 71 ± 7 | 3 ± 3 |
| CD8 T | 6 ± 5 | 2 ± 1 |
| CD56 NK | 6 ± 3 | 4 ± 2 |
| CD3 T | 79 ± 8 | 5 ± 3 |
The cultures were restimulated twice, on the 7th and 14th days with irradiated (50 Gy) autologous EBV-infected B cells. Half of the culture medium was replaced with fresh medium. From the 9th day, 20 U/mL IL2 was added every 3rd day. Results represent means plus or minus SD of 3 experiments.
LTB4 was added at the initiation of the culture.