Gene expression results by RT-PCR
| . | INSR . | IGF1R . | TNFSF10 . | NTRK2 . | ABCC11 . | ABCB1 . |
|---|---|---|---|---|---|---|
| HuT78 | 1 | 1 | 1 | 1 | 1 | 1 |
| DpVp35 | 390 ± 255 | 0.93 ± 0.30 | 0.52 ± 0.46 | 1.8 ± 0.63 | 0.71 ± 0.53 | 77 ± 31 |
| DpVp50 | 648 ± 262 | 0.69 ± 0.49 | 0.06 ± 0.06 | 1.6 ± 0.96 | 0.77 ± 0.06 | 4.1 ± 3.8 |
| DpP75 | 837 ± 202 | 0.65 ± 0.43 | 0.055 ± 0.057 | 0.48 ± 0.58 | 0.35 ± 0.02 | 14.4 ± 6.0 |
| INSR (fold increase) in HuT78 cells treated with romidepsin | ||||||
| Control | Dp 0.1 ng/mL | Dp 1 ng/mL | Dp 2 ng/mL | Dp 4 ng/mL | ||
| 1 | 0.99 ± 0.14 | 18.95 ± 6.01 | 34.25 ± 12.65 | 25.75 ± 5.86 | ||
| . | INSR . | IGF1R . | TNFSF10 . | NTRK2 . | ABCC11 . | ABCB1 . |
|---|---|---|---|---|---|---|
| HuT78 | 1 | 1 | 1 | 1 | 1 | 1 |
| DpVp35 | 390 ± 255 | 0.93 ± 0.30 | 0.52 ± 0.46 | 1.8 ± 0.63 | 0.71 ± 0.53 | 77 ± 31 |
| DpVp50 | 648 ± 262 | 0.69 ± 0.49 | 0.06 ± 0.06 | 1.6 ± 0.96 | 0.77 ± 0.06 | 4.1 ± 3.8 |
| DpP75 | 837 ± 202 | 0.65 ± 0.43 | 0.055 ± 0.057 | 0.48 ± 0.58 | 0.35 ± 0.02 | 14.4 ± 6.0 |
| INSR (fold increase) in HuT78 cells treated with romidepsin | ||||||
| Control | Dp 0.1 ng/mL | Dp 1 ng/mL | Dp 2 ng/mL | Dp 4 ng/mL | ||
| 1 | 0.99 ± 0.14 | 18.95 ± 6.01 | 34.25 ± 12.65 | 25.75 ± 5.86 | ||
Expression of various genes normalized to GAPDH levels as determined by RT-PCR. Gene expression values for resistant sublines were then divided by expression levels for parental cells to obtain the fold increase for each subline.