Table 1 qPCR array analysis of liver and... | American Society of Hematology

Table 1

qPCR array analysis of liver and spleen

Gene name	Gene symbols	Liver	Liver	Spleen	Spleen
Gene name	Gene symbols	Fold change*	P	Fold change*	P
Adiponectin, C1Q and collagen domain containing	Adipoq	0.64	.022826†	1.02	.870631
Advanced glycosylation end-product-specific receptor	Ager	0.64	.021826†	0.82	.241839
Annexin A1	Anxa1	0.89	.377359	1.59	.003136†
AXL receptor tyrosine kinase	Axl	0.93	.490164	0.94	.469823
Complement component 3	C3	1.28	.003722†	0.11	.259802
Calreticulin	Calr	1.46	.010282†	0.91	.37959
CD14 antigen	Cd14	2.38	.00056†	0.90	.640137
CD36 antigen	Cd36	0.79	.164021	0.98	.798815
CD44 antigen	Cd44	0.95	.572287	0.82	.208215
CD47 antigen (Rh-related antigen, integrin-associated signal transducer)	Cd47	0.86	.10013	1.77	.709099
Carcinoembryonic antigen-related cell adhesion molecule 3	Ceacam3	0.64	.021826†	1.02	.761682
C-type lectin domain family 7, member a	Clec7a	0.65	.000025†	0.97	.774483
Chloride intracellular channel 4 (mitochondrial)	Clic4	0.79	.002799†	0.97	.562036
Calponin 2	Cnn2	0.68	.003551†	0.80	.314429
Collectin subfamily member 12	Colec12	0.98	.735378	0.73	.01582†
V-crk sarcoma virus CT10 oncogene homolog (avian)	Crk	0.90	.298475	0.77	.027703†
C-reactive protein, pentraxin-related	Crp	1.31	.013305†	1.02	.761682
Colony-stimulating factor 1 (macrophage)	Csf1	1.33	.014056†	0.77	.043912†
Colony-stimulating factor 2 (granulocyte-macrophage)	Csf2	0.76	.554382	1.67	.777404
C-src tyrosine kinase	Csk	1.3	.370645	0.65	.172049
Cytochrome P450, family 2, subfamily s, polypeptide 1	Cyp2s1	0.61	.024005†	0.85	.576908
Dedicator of cytokinesis 1	Dock1	0.81	.024938†	0.92	.466954
Dedicator of cytokinesis 2	Dock2	0.95	.861987	0.73	.115206
Engulfment and cell motility 1, ced-12 homolog (C elegans)	Elmo1	0.76	.055912	0.70	.019194†
Fas (TNF receptor superfamily member 6)	Fas	1.29	.091078	0.75	.089305
Fc receptor, IgE, high-affinity I, γ polypeptide	Fcer1g	1.06	.609571	0.90	.477472
Fc receptor, IgG, high-affinity I	Fcgr1	1.49	.011813†	1.62	.091199
Fc receptor, IgG, low-affinity IIb	Fcgr2b	0.74	.012309†	0.77	.052798
Fc receptor, IgG, low-affinity III	Fcgr3	0.95	.42037	1.26	.018224†
Fyn proto-oncogene	Fyn	0.752	.023546†	0.71	.031913†
GULP, engulfment adaptor PTB domain containing 1	Gulp1	0.64	.021826†	1.02	.761682
Interferon γ	Ifng	0.64	.021826†	0.73	.439154
Interleukin 1 receptor-like 1	Il1rl1	0.64	.021826†	1.93	.077434
IQ motif and Sec7 domain 1	Iqsec1	1.18	.188148	0.65	.026589†
Integrin α M	Itgam	2.36	.002989†	1.63	.050069
Integrin α V	Itgav	0.93	.095144	0.76	.177878
Integrin β 2	Itgb2	0.81	.20262	0.91	.106374
Yamaguchi sarcoma viral (v-yes-1) oncogene homolog	Lyn	0.98	.710285	0.92	.082347
Mitogen-activated protein kinase 14	Mapk14	0.98	.675627	1	.911288
Macrophage receptor with collagenous structure	Marco	1.22	.109189	1.04	.577212
Mannose-binding lectin (protein C) 2	Mbl2	0.96	.67019	1.02	.761682
Mucolipin 3	Mcoln3	0.64	.021826†	0.83	.099829
C-mer proto-oncogene tyrosine kinase	Mertk	0.80	.009257†	0.98	.773705
Milk fat globule-EGF factor 8 protein	Mfge8	0.71	.000236†	0.67	.021755†
Macrophage migration inhibitory factor	Mif	0.56	.000498†	0.61	.02087†
Moesin	Msn	0.92	.239447	0.75	.094797
Myeloid differentiation primary response gene 88	Myd88	0.81	.097953	0.74	.087204
Nucleotide-binding oligomerization domain containing 1	Nod1	0.97	.854512	0.69	.040064†
Platelet/endothelial cell adhesion molecule 1	Pecam1	0.78	.003327†	0.82	.014935†
Phosphatidylinositol 3-kinase, catalytic, β polypeptide	Pik3cb	0.87	.010491†	1.03	.948485
Phosphatidylinositol-4-phosphate 5-kinase, type 1 α	Pip5k1a	0.64	.021826†	1.32	.120657
Phospholipase A2, group IVA (cytosolic, calcium-dependent)	Pla2g4a	1.45	.09121	1.81	.40386
Phospholipase A2, group V	Pla2g5	0.64	.021826†	1.01	.936996
Phospholipase D1	Pld1	1.15	.154763	0.86	.052754
Phospholipase D2	Pld2	1.17	.400898	0.83	.226317
Protein kinase C, ε	Prkce	0.99	.903633	0.64	.01899†
Protein S (α)	Pros1	1.37	.033477†	0.98	.8042
Phosphatase and tensin homolog	Pten	1.09	.277962	0.78	.115533
RAB5A, member RAS oncogene family	Rab5a	0.84	.000814†	0.77	.21849
RAB7, member RAS oncogene family	Rab7	0.79	.000643†	0.82	.237774
RAS-related C3 botulinum substrate 1	Rac1	0.82	.022787†	0.91	.050714
RAS-related C3 botulinum substrate 2	Rac2	1.13	.056517	0.76	.009861†
V-ral simian leukemia viral oncogene homolog A (ras related)	Rala	0.80	.190775	0.75	.016177†
V-ral simian leukemia viral oncogene homolog B (ras related)	Ralb	0.83	.050971	0.90	.123584
Rap guanine nucleotide exchange factor (GEF) 3	Rapgef3	0.54	.089669	0.88	.038894†
Ras homolog gene family, member A	Rhoa	0.73	.000332†	0.89	.180041
Scavenger receptor class B, member 1	Scarb1	1.14	.070894	1.05	.655597
Serine (or cysteine) peptidase inhibitor, clade E, member 1	Serpine1	0.70	.005876†	0.76	.277016
Surfactant-associated protein D	Sftpd	1.45	.04158†	1.02	.761682
Sialic acid-binding Ig-like lectin 1, sialoadhesin	Siglec1	1.25	.007915†	0.93	.537381
Signal-regulatory protein β 1A	Sirpb1a	2.48	.075493	1.04	.87213
Stabilin 2	Stab2	0.83	.000006†	0.85	.239987
Syntaxin 18	Stx18	1.15	.175211	0.85	.119873
Spleen tyrosine kinase	Sykb	1.32	.204417	0.57	.024911†
Transglutaminase 2, C polypeptide	Tgm2	1.33	.000057†	0.99	.894431
Toll-like receptor adaptor molecule 1	Ticam1	0.73	.059132	0.83	.251845
Toll-like receptor 3	Tlr3	1.40	.044291†	0.91	.544885
Toll-like receptor 9	Tlr9	1.04	.740029	0.79	.089971
Tumor necrosis factor	Tnf	0.65	.031306†	0.71	.057986
Tumor necrosis factor (ligand) superfamily, member 11	Tnfsf11	0.64	.021826†	1.08	.639823
Vesicle-associated membrane protein 7	Vamp7	0.98	.640446	0.92	.490228
Vav 1 oncogene	Vav1	1.01	.908941	0.7	.013642†
Wiskott-Aldrich syndrome homolog (human)	Was	0.85	.150303	0.74	.104693
Wingless-related MMTV integration site 5A	Wnt5a	0.86	.367722	0.52	.186013

Gene name	Gene symbols	Liver	Liver	Spleen	Spleen
Gene name	Gene symbols	Fold change*	P	Fold change*	P
Adiponectin, C1Q and collagen domain containing	Adipoq	0.64	.022826†	1.02	.870631
Advanced glycosylation end-product-specific receptor	Ager	0.64	.021826†	0.82	.241839
Annexin A1	Anxa1	0.89	.377359	1.59	.003136†
AXL receptor tyrosine kinase	Axl	0.93	.490164	0.94	.469823
Complement component 3	C3	1.28	.003722†	0.11	.259802
Calreticulin	Calr	1.46	.010282†	0.91	.37959
CD14 antigen	Cd14	2.38	.00056†	0.90	.640137
CD36 antigen	Cd36	0.79	.164021	0.98	.798815
CD44 antigen	Cd44	0.95	.572287	0.82	.208215
CD47 antigen (Rh-related antigen, integrin-associated signal transducer)	Cd47	0.86	.10013	1.77	.709099
Carcinoembryonic antigen-related cell adhesion molecule 3	Ceacam3	0.64	.021826†	1.02	.761682
C-type lectin domain family 7, member a	Clec7a	0.65	.000025†	0.97	.774483
Chloride intracellular channel 4 (mitochondrial)	Clic4	0.79	.002799†	0.97	.562036
Calponin 2	Cnn2	0.68	.003551†	0.80	.314429
Collectin subfamily member 12	Colec12	0.98	.735378	0.73	.01582†
V-crk sarcoma virus CT10 oncogene homolog (avian)	Crk	0.90	.298475	0.77	.027703†
C-reactive protein, pentraxin-related	Crp	1.31	.013305†	1.02	.761682
Colony-stimulating factor 1 (macrophage)	Csf1	1.33	.014056†	0.77	.043912†
Colony-stimulating factor 2 (granulocyte-macrophage)	Csf2	0.76	.554382	1.67	.777404
C-src tyrosine kinase	Csk	1.3	.370645	0.65	.172049
Cytochrome P450, family 2, subfamily s, polypeptide 1	Cyp2s1	0.61	.024005†	0.85	.576908
Dedicator of cytokinesis 1	Dock1	0.81	.024938†	0.92	.466954
Dedicator of cytokinesis 2	Dock2	0.95	.861987	0.73	.115206
Engulfment and cell motility 1, ced-12 homolog (C elegans)	Elmo1	0.76	.055912	0.70	.019194†
Fas (TNF receptor superfamily member 6)	Fas	1.29	.091078	0.75	.089305
Fc receptor, IgE, high-affinity I, γ polypeptide	Fcer1g	1.06	.609571	0.90	.477472
Fc receptor, IgG, high-affinity I	Fcgr1	1.49	.011813†	1.62	.091199
Fc receptor, IgG, low-affinity IIb	Fcgr2b	0.74	.012309†	0.77	.052798
Fc receptor, IgG, low-affinity III	Fcgr3	0.95	.42037	1.26	.018224†
Fyn proto-oncogene	Fyn	0.752	.023546†	0.71	.031913†
GULP, engulfment adaptor PTB domain containing 1	Gulp1	0.64	.021826†	1.02	.761682
Interferon γ	Ifng	0.64	.021826†	0.73	.439154
Interleukin 1 receptor-like 1	Il1rl1	0.64	.021826†	1.93	.077434
IQ motif and Sec7 domain 1	Iqsec1	1.18	.188148	0.65	.026589†
Integrin α M	Itgam	2.36	.002989†	1.63	.050069
Integrin α V	Itgav	0.93	.095144	0.76	.177878
Integrin β 2	Itgb2	0.81	.20262	0.91	.106374
Yamaguchi sarcoma viral (v-yes-1) oncogene homolog	Lyn	0.98	.710285	0.92	.082347
Mitogen-activated protein kinase 14	Mapk14	0.98	.675627	1	.911288
Macrophage receptor with collagenous structure	Marco	1.22	.109189	1.04	.577212
Mannose-binding lectin (protein C) 2	Mbl2	0.96	.67019	1.02	.761682
Mucolipin 3	Mcoln3	0.64	.021826†	0.83	.099829
C-mer proto-oncogene tyrosine kinase	Mertk	0.80	.009257†	0.98	.773705
Milk fat globule-EGF factor 8 protein	Mfge8	0.71	.000236†	0.67	.021755†
Macrophage migration inhibitory factor	Mif	0.56	.000498†	0.61	.02087†
Moesin	Msn	0.92	.239447	0.75	.094797
Myeloid differentiation primary response gene 88	Myd88	0.81	.097953	0.74	.087204
Nucleotide-binding oligomerization domain containing 1	Nod1	0.97	.854512	0.69	.040064†
Platelet/endothelial cell adhesion molecule 1	Pecam1	0.78	.003327†	0.82	.014935†
Phosphatidylinositol 3-kinase, catalytic, β polypeptide	Pik3cb	0.87	.010491†	1.03	.948485
Phosphatidylinositol-4-phosphate 5-kinase, type 1 α	Pip5k1a	0.64	.021826†	1.32	.120657
Phospholipase A2, group IVA (cytosolic, calcium-dependent)	Pla2g4a	1.45	.09121	1.81	.40386
Phospholipase A2, group V	Pla2g5	0.64	.021826†	1.01	.936996
Phospholipase D1	Pld1	1.15	.154763	0.86	.052754
Phospholipase D2	Pld2	1.17	.400898	0.83	.226317
Protein kinase C, ε	Prkce	0.99	.903633	0.64	.01899†
Protein S (α)	Pros1	1.37	.033477†	0.98	.8042
Phosphatase and tensin homolog	Pten	1.09	.277962	0.78	.115533
RAB5A, member RAS oncogene family	Rab5a	0.84	.000814†	0.77	.21849
RAB7, member RAS oncogene family	Rab7	0.79	.000643†	0.82	.237774
RAS-related C3 botulinum substrate 1	Rac1	0.82	.022787†	0.91	.050714
RAS-related C3 botulinum substrate 2	Rac2	1.13	.056517	0.76	.009861†
V-ral simian leukemia viral oncogene homolog A (ras related)	Rala	0.80	.190775	0.75	.016177†
V-ral simian leukemia viral oncogene homolog B (ras related)	Ralb	0.83	.050971	0.90	.123584
Rap guanine nucleotide exchange factor (GEF) 3	Rapgef3	0.54	.089669	0.88	.038894†
Ras homolog gene family, member A	Rhoa	0.73	.000332†	0.89	.180041
Scavenger receptor class B, member 1	Scarb1	1.14	.070894	1.05	.655597
Serine (or cysteine) peptidase inhibitor, clade E, member 1	Serpine1	0.70	.005876†	0.76	.277016
Surfactant-associated protein D	Sftpd	1.45	.04158†	1.02	.761682
Sialic acid-binding Ig-like lectin 1, sialoadhesin	Siglec1	1.25	.007915†	0.93	.537381
Signal-regulatory protein β 1A	Sirpb1a	2.48	.075493	1.04	.87213
Stabilin 2	Stab2	0.83	.000006†	0.85	.239987
Syntaxin 18	Stx18	1.15	.175211	0.85	.119873
Spleen tyrosine kinase	Sykb	1.32	.204417	0.57	.024911†
Transglutaminase 2, C polypeptide	Tgm2	1.33	.000057†	0.99	.894431
Toll-like receptor adaptor molecule 1	Ticam1	0.73	.059132	0.83	.251845
Toll-like receptor 3	Tlr3	1.40	.044291†	0.91	.544885
Toll-like receptor 9	Tlr9	1.04	.740029	0.79	.089971
Tumor necrosis factor	Tnf	0.65	.031306†	0.71	.057986
Tumor necrosis factor (ligand) superfamily, member 11	Tnfsf11	0.64	.021826†	1.08	.639823
Vesicle-associated membrane protein 7	Vamp7	0.98	.640446	0.92	.490228
Vav 1 oncogene	Vav1	1.01	.908941	0.7	.013642†
Wiskott-Aldrich syndrome homolog (human)	Was	0.85	.150303	0.74	.104693
Wingless-related MMTV integration site 5A	Wnt5a	0.86	.367722	0.52	.186013

Fold change (2^−ΔΔCT) is the normalized gene expression (2^−ΔCT) in the Plg^−/− samples divided by the normalized gene expression (2^−ΔCT) in the Plg^+/+ samples. Therefore, fold-change values <1 indicate a downregulation.

†

P value <.05.

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