Recommendations for monitoring individual patients.
| *The Sokal score28 is calculated in patients at the time of diagnosis by using a specific formula that takes into account their age, spleen size (in cm below the costal margin), platelet count (‘ 109/L) and percentage of myeloid blast cells in the blood. The score for a given patient can be obtained very rapidly by accessing www.roc.se/Sokal.asp. |
| FISH indicates fluorescent in situ hybridization; RQ-PCR, real time quantitative polymerase chain reaction. |
| At diagnosis |
| Full blood count and 100 cell differential |
| Calculation of Sokal score* |
| Bone marrow aspirate with cytogenetic analysis of 30 metaphases |
| [FISH for BCR-ABL1 in the absence of a Philadelphia chromosome] |
| RQ-PCR for BCR-ABL1 transcripts to characterize the BCR-ABL1 junction [Optional] |
| Thereafter: |
| Blood counts at intervals of two or more weeks |
| Liver chemistry |
| At 3 months |
| Blood count |
| Bone marrow cytogenetics |
| RQ-PCR for BCR-ABL1 transcripts |
| At 6 months |
| Blood count |
| Bone marrow cytogenetics |
| RQ-PCR for BCR-ABL1 transcripts |
| [Thereafter bone marrow aspirates are only required if CCyR has not been achieved] |
| At 3 month intervals thereafter: |
| RQ-PCR for BCR-ABL1 transcripts indefinitely |
| *The Sokal score28 is calculated in patients at the time of diagnosis by using a specific formula that takes into account their age, spleen size (in cm below the costal margin), platelet count (‘ 109/L) and percentage of myeloid blast cells in the blood. The score for a given patient can be obtained very rapidly by accessing www.roc.se/Sokal.asp. |
| FISH indicates fluorescent in situ hybridization; RQ-PCR, real time quantitative polymerase chain reaction. |
| At diagnosis |
| Full blood count and 100 cell differential |
| Calculation of Sokal score* |
| Bone marrow aspirate with cytogenetic analysis of 30 metaphases |
| [FISH for BCR-ABL1 in the absence of a Philadelphia chromosome] |
| RQ-PCR for BCR-ABL1 transcripts to characterize the BCR-ABL1 junction [Optional] |
| Thereafter: |
| Blood counts at intervals of two or more weeks |
| Liver chemistry |
| At 3 months |
| Blood count |
| Bone marrow cytogenetics |
| RQ-PCR for BCR-ABL1 transcripts |
| At 6 months |
| Blood count |
| Bone marrow cytogenetics |
| RQ-PCR for BCR-ABL1 transcripts |
| [Thereafter bone marrow aspirates are only required if CCyR has not been achieved] |
| At 3 month intervals thereafter: |
| RQ-PCR for BCR-ABL1 transcripts indefinitely |