Inhibitory activity of YO compounds on binding site mutants
| μPlm binding site mutants . | Activity (Km) (μM) . | IC50 (μM) . | |
|---|---|---|---|
| YO-2 . | PSI-112 . | ||
| Wild-type | 227 ± 8.3 | 0.24 ± 0.004 | 0.38 ± 0.010 |
| F587A | 326.5 ± 9.8 | 1.15 ± 0.044 | 1.48 ± 0.037 |
| K607A | 266.6 ± 16.3 | 0.17 ± 0.006 | 0.37 ± 0.016 |
| F587A/K607A | 321.4 ± 17.7 | 1.10 ± 0.038 | 1.75 ± 0.066 |
| μPlm binding site mutants . | Activity (Km) (μM) . | IC50 (μM) . | |
|---|---|---|---|
| YO-2 . | PSI-112 . | ||
| Wild-type | 227 ± 8.3 | 0.24 ± 0.004 | 0.38 ± 0.010 |
| F587A | 326.5 ± 9.8 | 1.15 ± 0.044 | 1.48 ± 0.037 |
| K607A | 266.6 ± 16.3 | 0.17 ± 0.006 | 0.37 ± 0.016 |
| F587A/K607A | 321.4 ± 17.7 | 1.10 ± 0.038 | 1.75 ± 0.066 |
Data are the mean and standard error derived from 3 or more independent experiments (see supplemental Data for details).