Selected components of the human platelet PtdIns(3,4,5)P3 interactome
| Accession No. . | Gene name . | Protein description . | Mean area . | Mean score . | Mean coverage (%) . | Mean peptides . | Mean PSM . | Times identified, n/3 . | Characterized in platelets? . |
|---|---|---|---|---|---|---|---|---|---|
| Q06187 | BTK | Tyrosine-protein kinase BTK | 8.56e+9 | 3109.1 | 75.4 | 61 | 1228 | 3 | Yes |
| Q14644 | RASA3 | Ras GTPase-activating protein 3 | 4.58e+9 | 2083.6 | 77.3 | 70 | 953 | 3 | Yes |
| Q9UN19 | DAPP1 | Dual adapter for phosphotyrosine and 3-phosphoinositides/Bam32 | 2.24e+9 | 777.1 | 80.2 | 24 | 325 | 3 | No |
| P42680 | TEC | Tyrosine-protein kinase Tec | 1.02e+9 | 522.7 | 67.2 | 47 | 253 | 3 | Yes |
| Q99418 | CYTH2 | Cytohesin-2/ARNO | 9.58e+8 | 384.3 | 53.0 | 23 | 165 | 3 | Yes |
| O43739 | CYTH3 | Cytohesin-3/GRP1 | 8.02e+8 | 132.1 | 32.4 | 14 | 61 | 3 | No |
| Q15438 | CYTH1 | Cytohesin-1/PSCD1 | 7.12e+8 | 171.5 | 39.9 | 17 | 79 | 3 | No |
| Q15283 | RASA2 | Ras GTPase-activating protein 2 | 4.49e+8 | 131.0 | 34.7 | 27 | 76 | 3 | No |
| Q9Y2L6 | FRMD4B | FERM domain–containing protein 4B/GRSP1 | 3.26e+8 | 554.8 | 32.0 | 28 | 230 | 3 | No |
| O95782 | AP2A1 | AP-2 complex subunit α-1 | 2.96e+8 | 202.3 | 45.4 | 42 | 102 | 3 | No |
| Q92556 | ELMO1 | Engulfment and cell motility protein 1 | 1.11e+8 | 131.2 | 37.4 | 28 | 61 | 3 | No |
| Q15027 | ACAP1 | Arf-GAP with coiled-coil, ANK repeat, and PH domain–containing protein 1/CENTB1 | 8.56e+7 | 133.8 | 40.7 | 25 | 55 | 3 | No |
| P42566 | EPS15 | Epidermal growth factor receptor substrate 15 | 8.44e+7 | 148.4 | 39.0 | 31 | 68 | 3 | No |
| Q9H7D0 | DOCK5 | Dedicator of cytokinesis protein 5 | 6.62e+7 | 123.7 | 25.8 | 46 | 69 | 3 | No |
| Q86UU1 | PHLDB1 | PH-like domain family B member 1/LL5α | 5.95e+7 | 106.9 | 23.0 | 32 | 55 | 3 | No |
| Q8WWN9 | IPCEF1 | Interactor protein for cytohesin exchange factors 1 | 5.82e+7 | 69.7 | 31.4 | 13 | 29 | 3 | No |
| Q96JJ3 | ELMO2 | Engulfment and cell motility protein 2 | 5.72e+7 | 24.9 | 16.4 | 11 | 17 | 3 | No |
| Q14185 | DOCK1 | Dedicator of cytokinesis protein 1 | 4.96e+7 | 75.8 | 18.7 | 33 | 44 | 3 | No |
| A0FGR8 | ESYT2 | Extended synaptotagmin-2 | 4.86e+7 | 116.0 | 30.3 | 23 | 54 | 3 | No |
| O75689 | ADAP1 | Arf-GAP with dual PH domain–containing protein 1/CENTA1 | 3.85e+7 | 42.1 | 32.9 | 13 | 21 | 3 | No |
| O15530 | PDPK1 | 3-phosphoinositide–dependent protein kinase 1/PDK1 | 2.69e+7 | 46.3 | 35.9 | 17 | 25 | 3 | Yes |
| P07900 | HSP90AA1 | Heat shock protein HSP 90-α | 2.26e+7 | 50.6 | 21.9 | 16 | 27 | 3 | Yes |
| Q8WWW8 | GAB3 | GRB2-associated–binding protein 3 | 1.65e+7 | 37.1 | 20.6 | 11 | 18 | 3 | No |
| Q9ULP9 | TBC1D24 | TBC1 domain family member 24 | 1.53e+7 | 18.8 | 17.4 | 9 | 11 | 3 | No |
| Q5TC63 | GRTP1 | Growth hormone–regulated TBC protein 1/TBC1D6 | 1.07e+7 | 14.2 | 17.7 | 5 | 8 | 3 | No |
| O75563 | SKAP2 | Src kinase–associated phosphoprotein 2/SCAP2 | 1.00e+7 | 7.3 | 13.3 | 4 | 4 | 3 | Yes |
| Q92608 | DOCK2 | Dedicator of cytokinesis protein 2 | 9.28e+6 | 6.5 | 2.7 | 5 | 5 | 2 | No |
| B0I1T2 | MYO1G | Unconventional myosin-1G | 8.90e+6 | 24.8 | 14.5 | 13 | 16 | 3 | No |
| Q15057 | ACAP2 | Arf-GAP with coiled-coil, ANK repeat, and PH domain–containing protein 2/CENTB2 | 8.77e+6 | 40.3 | 19.5 | 12 | 18 | 3 | No |
| Q9Y5X1 | SNX9 | Sorting nexin-9 | 8.48e+6 | 14.1 | 13.5 | 6 | 8 | 3 | No |
| O00159 | MYO1C | Unconventional myosin-Ic | 7.22e+6 | 15.6 | 9.3 | 8 | 9 | 3 | No |
| Q9UQC2 | GAB2 | GRB2-associated–binding protein 2 | 7.19e+6 | 34.0 | 20.5 | 11 | 19 | 3 | Yes |
| O75791 | GRAP2 | GRB2-related adapter protein 2/GADS | 4.40e+6 | 9.9 | 18.4 | 5 | 5 | 3 | Yes |
| P98082 | DAB2 | Disabled homolog 2 | 3.37e+6 | 10.1 | 8.5 | 5 | 5 | 3 | Yes |
| P31751 | AKT2 | RAC-β serine/threonine-protein kinase/PKB β | 2.95e+6 | 10.2 | 12.1 | 5 | 6 | 3 | Yes |
| Q9UPU7 | TBC1D2B | TBC1 domain family member 2B | 2.16e+6 | 15.6 | 10.8 | 9 | 10 | 3 | No |
| Q14155 | ARHGEF7 | Rho guanine nucleotide exchange factor 7 | 2.12e+6 | 6.8 | 5.8 | 5 | 5 | 3 | No |
| Q0JRZ9 | FCHO2 | F-BAR domain only protein 2 | 1.80e+6 | 10.8 | 7.9 | 5 | 7 | 3 | No |
| P31749 | AKT1 | RAC-α serine/threonine-protein kinase/PKB α | 1.77e+6 | 1.8 | 3.3 | 1 | 1 | 3 | Yes |
| Q12965 | MYO1E | Unconventional myosin-Ie | 1.73e+6 | 10.0 | 5.2 | 5 | 5 | 3 | No |
| Q96HS1 | PGAM5 | Serine/threonine-protein phosphatase PGAM5 | 1.27e+6 | 3.4 | 9.7 | 3 | 4 | 3 | No |
| Q8NF50 | DOCK8 | Dedicator of cytokinesis protein 8 | 1.14e+6 | 8.0 | 2.4 | 4 | 4 | 3 | No |
| Q8NEU8 | APPL2 | DCC-interacting protein 13-β/DIP13b/APPL2 | 1.13e+6 | 7.8 | 7.1 | 4 | 4 | 3 | No |
| Q13480 | GAB1 | GRB2-associated–binding protein 1 | 8.18e+5 | 5.4 | 3.3 | 2 | 3 | 3 | Yes |
| Q6P1M0 | SLC27A4 | Long-chain fatty acid transport protein 4 | 7.11e+5 | 10.3 | 10.2 | 6 | 6 | 3 | No |
| O43182 | ARHGAP6 | Rho GTPase-activating protein 6 | 6.48e+5 | 7.7 | 7.5 | 5 | 5 | 2 | No |
| Q96N67 | DOCK7 | Dedicator of cytokinesis protein 7 | 6.27e+5 | 6.7 | 1.9 | 3 | 3 | 3 | No |
| Q9Y2X7 | GIT1 | ARF GTPase-activating protein GIT1 | 5.83e+5 | 4.6 | 2.5 | 2 | 2 | 3 | Yes |
| P52306 | RAP1GDS1 | Rap1 GTPase-GDP dissociation stimulator 1/GDS1 | 2.76e+5 | 2.4 | 2.0 | 1 | 1 | 3 | No |
| Q96P48 | ARAP1 | Arf-GAP with Rho-GAP domain, ANK repeat, and PH domain–containing protein 1/CENTD2 | 2.38e+6 | 22.3 | 8.6 | 11 | 11 | 2 | No |
| O00160 | MYO1F | Unconventional myosin-If | 1.84e+6 | 13.4 | 8.3 | 8 | 8 | 2 | No |
| O95379 | TNFAIP8 | Tumor necrosis factor α–induced protein 8 | 1.12e+6 | 0.9 | 4.0 | 1 | 1 | 2 | No |
| Q8WVP5 | TNFAIP8L1 | Tumor necrosis factor α–induced protein 8–like protein 1/TIPE1 | 3.18e+5 | 4.1 | 14.3 | 2 | 2 | 2 | No |
| Q9BPZ7 | MAPKAP1 | Target of rapamycin complex 2 subunit MAPKAP1/SIN1 | 2.93e+5 | 0.9 | 2.1 | 1 | 1 | 2 | No |
| Accession No. . | Gene name . | Protein description . | Mean area . | Mean score . | Mean coverage (%) . | Mean peptides . | Mean PSM . | Times identified, n/3 . | Characterized in platelets? . |
|---|---|---|---|---|---|---|---|---|---|
| Q06187 | BTK | Tyrosine-protein kinase BTK | 8.56e+9 | 3109.1 | 75.4 | 61 | 1228 | 3 | Yes |
| Q14644 | RASA3 | Ras GTPase-activating protein 3 | 4.58e+9 | 2083.6 | 77.3 | 70 | 953 | 3 | Yes |
| Q9UN19 | DAPP1 | Dual adapter for phosphotyrosine and 3-phosphoinositides/Bam32 | 2.24e+9 | 777.1 | 80.2 | 24 | 325 | 3 | No |
| P42680 | TEC | Tyrosine-protein kinase Tec | 1.02e+9 | 522.7 | 67.2 | 47 | 253 | 3 | Yes |
| Q99418 | CYTH2 | Cytohesin-2/ARNO | 9.58e+8 | 384.3 | 53.0 | 23 | 165 | 3 | Yes |
| O43739 | CYTH3 | Cytohesin-3/GRP1 | 8.02e+8 | 132.1 | 32.4 | 14 | 61 | 3 | No |
| Q15438 | CYTH1 | Cytohesin-1/PSCD1 | 7.12e+8 | 171.5 | 39.9 | 17 | 79 | 3 | No |
| Q15283 | RASA2 | Ras GTPase-activating protein 2 | 4.49e+8 | 131.0 | 34.7 | 27 | 76 | 3 | No |
| Q9Y2L6 | FRMD4B | FERM domain–containing protein 4B/GRSP1 | 3.26e+8 | 554.8 | 32.0 | 28 | 230 | 3 | No |
| O95782 | AP2A1 | AP-2 complex subunit α-1 | 2.96e+8 | 202.3 | 45.4 | 42 | 102 | 3 | No |
| Q92556 | ELMO1 | Engulfment and cell motility protein 1 | 1.11e+8 | 131.2 | 37.4 | 28 | 61 | 3 | No |
| Q15027 | ACAP1 | Arf-GAP with coiled-coil, ANK repeat, and PH domain–containing protein 1/CENTB1 | 8.56e+7 | 133.8 | 40.7 | 25 | 55 | 3 | No |
| P42566 | EPS15 | Epidermal growth factor receptor substrate 15 | 8.44e+7 | 148.4 | 39.0 | 31 | 68 | 3 | No |
| Q9H7D0 | DOCK5 | Dedicator of cytokinesis protein 5 | 6.62e+7 | 123.7 | 25.8 | 46 | 69 | 3 | No |
| Q86UU1 | PHLDB1 | PH-like domain family B member 1/LL5α | 5.95e+7 | 106.9 | 23.0 | 32 | 55 | 3 | No |
| Q8WWN9 | IPCEF1 | Interactor protein for cytohesin exchange factors 1 | 5.82e+7 | 69.7 | 31.4 | 13 | 29 | 3 | No |
| Q96JJ3 | ELMO2 | Engulfment and cell motility protein 2 | 5.72e+7 | 24.9 | 16.4 | 11 | 17 | 3 | No |
| Q14185 | DOCK1 | Dedicator of cytokinesis protein 1 | 4.96e+7 | 75.8 | 18.7 | 33 | 44 | 3 | No |
| A0FGR8 | ESYT2 | Extended synaptotagmin-2 | 4.86e+7 | 116.0 | 30.3 | 23 | 54 | 3 | No |
| O75689 | ADAP1 | Arf-GAP with dual PH domain–containing protein 1/CENTA1 | 3.85e+7 | 42.1 | 32.9 | 13 | 21 | 3 | No |
| O15530 | PDPK1 | 3-phosphoinositide–dependent protein kinase 1/PDK1 | 2.69e+7 | 46.3 | 35.9 | 17 | 25 | 3 | Yes |
| P07900 | HSP90AA1 | Heat shock protein HSP 90-α | 2.26e+7 | 50.6 | 21.9 | 16 | 27 | 3 | Yes |
| Q8WWW8 | GAB3 | GRB2-associated–binding protein 3 | 1.65e+7 | 37.1 | 20.6 | 11 | 18 | 3 | No |
| Q9ULP9 | TBC1D24 | TBC1 domain family member 24 | 1.53e+7 | 18.8 | 17.4 | 9 | 11 | 3 | No |
| Q5TC63 | GRTP1 | Growth hormone–regulated TBC protein 1/TBC1D6 | 1.07e+7 | 14.2 | 17.7 | 5 | 8 | 3 | No |
| O75563 | SKAP2 | Src kinase–associated phosphoprotein 2/SCAP2 | 1.00e+7 | 7.3 | 13.3 | 4 | 4 | 3 | Yes |
| Q92608 | DOCK2 | Dedicator of cytokinesis protein 2 | 9.28e+6 | 6.5 | 2.7 | 5 | 5 | 2 | No |
| B0I1T2 | MYO1G | Unconventional myosin-1G | 8.90e+6 | 24.8 | 14.5 | 13 | 16 | 3 | No |
| Q15057 | ACAP2 | Arf-GAP with coiled-coil, ANK repeat, and PH domain–containing protein 2/CENTB2 | 8.77e+6 | 40.3 | 19.5 | 12 | 18 | 3 | No |
| Q9Y5X1 | SNX9 | Sorting nexin-9 | 8.48e+6 | 14.1 | 13.5 | 6 | 8 | 3 | No |
| O00159 | MYO1C | Unconventional myosin-Ic | 7.22e+6 | 15.6 | 9.3 | 8 | 9 | 3 | No |
| Q9UQC2 | GAB2 | GRB2-associated–binding protein 2 | 7.19e+6 | 34.0 | 20.5 | 11 | 19 | 3 | Yes |
| O75791 | GRAP2 | GRB2-related adapter protein 2/GADS | 4.40e+6 | 9.9 | 18.4 | 5 | 5 | 3 | Yes |
| P98082 | DAB2 | Disabled homolog 2 | 3.37e+6 | 10.1 | 8.5 | 5 | 5 | 3 | Yes |
| P31751 | AKT2 | RAC-β serine/threonine-protein kinase/PKB β | 2.95e+6 | 10.2 | 12.1 | 5 | 6 | 3 | Yes |
| Q9UPU7 | TBC1D2B | TBC1 domain family member 2B | 2.16e+6 | 15.6 | 10.8 | 9 | 10 | 3 | No |
| Q14155 | ARHGEF7 | Rho guanine nucleotide exchange factor 7 | 2.12e+6 | 6.8 | 5.8 | 5 | 5 | 3 | No |
| Q0JRZ9 | FCHO2 | F-BAR domain only protein 2 | 1.80e+6 | 10.8 | 7.9 | 5 | 7 | 3 | No |
| P31749 | AKT1 | RAC-α serine/threonine-protein kinase/PKB α | 1.77e+6 | 1.8 | 3.3 | 1 | 1 | 3 | Yes |
| Q12965 | MYO1E | Unconventional myosin-Ie | 1.73e+6 | 10.0 | 5.2 | 5 | 5 | 3 | No |
| Q96HS1 | PGAM5 | Serine/threonine-protein phosphatase PGAM5 | 1.27e+6 | 3.4 | 9.7 | 3 | 4 | 3 | No |
| Q8NF50 | DOCK8 | Dedicator of cytokinesis protein 8 | 1.14e+6 | 8.0 | 2.4 | 4 | 4 | 3 | No |
| Q8NEU8 | APPL2 | DCC-interacting protein 13-β/DIP13b/APPL2 | 1.13e+6 | 7.8 | 7.1 | 4 | 4 | 3 | No |
| Q13480 | GAB1 | GRB2-associated–binding protein 1 | 8.18e+5 | 5.4 | 3.3 | 2 | 3 | 3 | Yes |
| Q6P1M0 | SLC27A4 | Long-chain fatty acid transport protein 4 | 7.11e+5 | 10.3 | 10.2 | 6 | 6 | 3 | No |
| O43182 | ARHGAP6 | Rho GTPase-activating protein 6 | 6.48e+5 | 7.7 | 7.5 | 5 | 5 | 2 | No |
| Q96N67 | DOCK7 | Dedicator of cytokinesis protein 7 | 6.27e+5 | 6.7 | 1.9 | 3 | 3 | 3 | No |
| Q9Y2X7 | GIT1 | ARF GTPase-activating protein GIT1 | 5.83e+5 | 4.6 | 2.5 | 2 | 2 | 3 | Yes |
| P52306 | RAP1GDS1 | Rap1 GTPase-GDP dissociation stimulator 1/GDS1 | 2.76e+5 | 2.4 | 2.0 | 1 | 1 | 3 | No |
| Q96P48 | ARAP1 | Arf-GAP with Rho-GAP domain, ANK repeat, and PH domain–containing protein 1/CENTD2 | 2.38e+6 | 22.3 | 8.6 | 11 | 11 | 2 | No |
| O00160 | MYO1F | Unconventional myosin-If | 1.84e+6 | 13.4 | 8.3 | 8 | 8 | 2 | No |
| O95379 | TNFAIP8 | Tumor necrosis factor α–induced protein 8 | 1.12e+6 | 0.9 | 4.0 | 1 | 1 | 2 | No |
| Q8WVP5 | TNFAIP8L1 | Tumor necrosis factor α–induced protein 8–like protein 1/TIPE1 | 3.18e+5 | 4.1 | 14.3 | 2 | 2 | 2 | No |
| Q9BPZ7 | MAPKAP1 | Target of rapamycin complex 2 subunit MAPKAP1/SIN1 | 2.93e+5 | 0.9 | 2.1 | 1 | 1 | 2 | No |
Presented are mean values from LC-MS/MS analysis of protein capture on PtdIns(3,4,5)P3-coupled beads across 3 independent donors. Proteins are ranked based on mean abundance. Shown are a spectrum of proteins identified in the screen, with the full data set presented in supplemental Table 1. Assessment of previous functional characterization in human or mouse platelets was carried out by literature searching. The final 5 proteins have previously reported PtdIns(3,4,5)P3 affinity and were captured on the PtdIns(3,4,5)P3 beads with lower stringency (see “Results”).
Area, T3PQ; score, the total score of the protein, which is the sum of all peptide XCorr values for that protein above the specified score threshold; coverage, the percentage of the protein sequence covered by the identified peptides; peptides, the number of unique peptide sequences identified for the protein; PSM, the total number of identified peptide sequences for the protein.