Phenotypical characterization of adult Hephint and control mice
| Genotype (sex) . | Body weight* (g) . | Hb (g/L) . | Hct (%) . | Liver nonheme iron†‡ (µg/mg tissue) . |
|---|---|---|---|---|
| Hephfl/fl (female) | 20.91 ± 1.48 (12)§ | 140.06 ± 10.04 (12) | 45.92 ± 6.77 (12) | 48.51 ± 21.82 (17) |
| Hephint/int (female KO) | 20.16 ± 1.91 (12) | 140.27 ± 10.93 (12) | 46.33 ± 5.25 (12) | 39.33 ± 15.68 (17) |
| Hephfl/y (male) | 27.22 ± 2.72 (12) | 130.78 ± 10.95 (12) | 45.16 ± 6.38 (12) | 38.11 ± 5.91 (17) |
| Hephint/y (male KO) | 26.75 ± 3.85 (12) | 130.95 ± 10.79 (12) | 48.71 ± 7.27 (12) | 30.40 ± 8.19 (14) |
| Genotype (sex) . | Body weight* (g) . | Hb (g/L) . | Hct (%) . | Liver nonheme iron†‡ (µg/mg tissue) . |
|---|---|---|---|---|
| Hephfl/fl (female) | 20.91 ± 1.48 (12)§ | 140.06 ± 10.04 (12) | 45.92 ± 6.77 (12) | 48.51 ± 21.82 (17) |
| Hephint/int (female KO) | 20.16 ± 1.91 (12) | 140.27 ± 10.93 (12) | 46.33 ± 5.25 (12) | 39.33 ± 15.68 (17) |
| Hephfl/y (male) | 27.22 ± 2.72 (12) | 130.78 ± 10.95 (12) | 45.16 ± 6.38 (12) | 38.11 ± 5.91 (17) |
| Hephint/y (male KO) | 26.75 ± 3.85 (12) | 130.95 ± 10.79 (12) | 48.71 ± 7.27 (12) | 30.40 ± 8.19 (14) |
| . | Serum nonheme iron‖(µg/dL) . | Iron absorption¶(% of dose) . | Enterocyte FOX activity#(AUC−1[ΔA570/Δt])** . | Serum Cp activity††(slope) . |
|---|---|---|---|---|
| Hephfl/fl (female) | 8.34 ± 2.61 (10) | 31.23 ± 13.37 (5) | 1.00 ± 0.01 (10) | 1.00 ± 0.03 (11) |
| Hephint/int (female KO) | 9.16 ± 3.14 (11) | 29.33 ± 19.43 (8) | 2.17 ± 0.18 (6) | 0.88 ± 0.30 (9) |
| Hephfl/y (male) | 6.23 ± 1.55 (10) | 15.48 ± 6.97 (7) | 1.01 ± 0.16 (5) | 0.83 ± 0.21 (11) |
| Hephint/y (male KO) | 7.21 ± 2.09 (11) | 18.52 ± 9.29 (11) | 1.89 ± 0.23 (6) | 0.68 ± 0.16 (7) |
| . | Serum nonheme iron‖(µg/dL) . | Iron absorption¶(% of dose) . | Enterocyte FOX activity#(AUC−1[ΔA570/Δt])** . | Serum Cp activity††(slope) . |
|---|---|---|---|---|
| Hephfl/fl (female) | 8.34 ± 2.61 (10) | 31.23 ± 13.37 (5) | 1.00 ± 0.01 (10) | 1.00 ± 0.03 (11) |
| Hephint/int (female KO) | 9.16 ± 3.14 (11) | 29.33 ± 19.43 (8) | 2.17 ± 0.18 (6) | 0.88 ± 0.30 (9) |
| Hephfl/y (male) | 6.23 ± 1.55 (10) | 15.48 ± 6.97 (7) | 1.01 ± 0.16 (5) | 0.83 ± 0.21 (11) |
| Hephint/y (male KO) | 7.21 ± 2.09 (11) | 18.52 ± 9.29 (11) | 1.89 ± 0.23 (6) | 0.68 ± 0.16 (7) |
Various physiological parameters were assessed (as indicated in the first row of each half of the table) in 10- to 12-wk-old experimental mice under physiological conditions. Values are means ± standard deviations (SDs) analyzed by 2-way ANOVA. No 2-way interactions were noted.
Sex main effect (P < .001).
Sex main effect (P = .01).
Genotype main effect (P = .0236).
Numbers in parentheses to the right of experimental values indicate number of mice in each group.
Sex main effect (P = .0423).
Sex main effect (P = .0423).
Genotype main effect (P < .0001).
AUC−1 (change in absorbance [A570]/change in time [t]).
Sex main effect (P = .0072).