Fe and Cu concentrations in liver and serum of adult, neonatal, and pregnant mice
| Experimental mice . | Liver Fe . | Serum Fe . | Liver Cu . | Serum Cu . |
|---|---|---|---|---|
| Adult Hephfl/fl | 553.33 ± 212.063 | 12.98 ± 3.48 | 12.97 ± 2.024 | 0.63 ± 0.075,* |
| Adult Hephint/int | 283.20 ± 58.1914,* | 12.06 ± 5.89 | 14.93 ± 1.39 | 0.56 ± 0.05 |
| Adult Hephfl/y | 416 ± 54.01 | 11 ± 4.01 | 16.55 ± 0.84 | 0.63 ± 0.07 |
| Adult Hephint/y | 290.83 ± 85.34 | 19.03 ± 11.29 | 16.27 ± 2.23 | 0.53 ± 0.10* |
| Weanling Hephfl/fl | a540.17 ± 203.521,6 | 17.48 ± 4.257 | 22.90 ± 7.28 | 0.62 ± 0.058,9 |
| Weanling Hephint/int | b294.67 ± 48.78 | 7.06 ± 1.94 | 26.42 ± 9.24 | 0.55 ± 0.04 |
| Weanling Hephfl/y | b321.50 ± 25.72 | 16.42 ± 6.47* | 25.80 ± 5.12 | 0.56 ± 0.06* |
| Weanling Hephint/y | b235.83 ± 151.00 | 11.83 ± 3.03 | 24.97 ± 3.37 | 0.51 ± 0.06* |
| Nonpregnant Hephfl/fl | a553.33 ± 212.062,10 | 12.98 ± 3.48 | 12.97 ± 2.0211 | 0.63 ± 0.0712,13 |
| Nonpregnant Hephint/int | b283.20 ± 58.19 | 12.06 ± 5.89* | 14.93 ± 1.39 | 0.56 ± 0.05 |
| Pregnant Hephfl/fl | b340.20 ± 70.70* | 10.80 ± 2.31* | 19.77 ± 2.41 | 2.11 ± 0.11** |
| Pregnant Hephint/int | c72.17 ± 11.55 | 8.78 ± 3.67 | 19.62 ± 2.52 | 1.90 ± 0.13** |
| Experimental mice . | Liver Fe . | Serum Fe . | Liver Cu . | Serum Cu . |
|---|---|---|---|---|
| Adult Hephfl/fl | 553.33 ± 212.063 | 12.98 ± 3.48 | 12.97 ± 2.024 | 0.63 ± 0.075,* |
| Adult Hephint/int | 283.20 ± 58.1914,* | 12.06 ± 5.89 | 14.93 ± 1.39 | 0.56 ± 0.05 |
| Adult Hephfl/y | 416 ± 54.01 | 11 ± 4.01 | 16.55 ± 0.84 | 0.63 ± 0.07 |
| Adult Hephint/y | 290.83 ± 85.34 | 19.03 ± 11.29 | 16.27 ± 2.23 | 0.53 ± 0.10* |
| Weanling Hephfl/fl | a540.17 ± 203.521,6 | 17.48 ± 4.257 | 22.90 ± 7.28 | 0.62 ± 0.058,9 |
| Weanling Hephint/int | b294.67 ± 48.78 | 7.06 ± 1.94 | 26.42 ± 9.24 | 0.55 ± 0.04 |
| Weanling Hephfl/y | b321.50 ± 25.72 | 16.42 ± 6.47* | 25.80 ± 5.12 | 0.56 ± 0.06* |
| Weanling Hephint/y | b235.83 ± 151.00 | 11.83 ± 3.03 | 24.97 ± 3.37 | 0.51 ± 0.06* |
| Nonpregnant Hephfl/fl | a553.33 ± 212.062,10 | 12.98 ± 3.48 | 12.97 ± 2.0211 | 0.63 ± 0.0712,13 |
| Nonpregnant Hephint/int | b283.20 ± 58.19 | 12.06 ± 5.89* | 14.93 ± 1.39 | 0.56 ± 0.05 |
| Pregnant Hephfl/fl | b340.20 ± 70.70* | 10.80 ± 2.31* | 19.77 ± 2.41 | 2.11 ± 0.11** |
| Pregnant Hephint/int | c72.17 ± 11.55 | 8.78 ± 3.67 | 19.62 ± 2.52 | 1.90 ± 0.13** |
Total iron and copper concentrations in liver and serum samples from experimental mice were determined by inductively coupled plasma mass spectrometry. Each sample was run in duplicate, and values were averaged. Values represent means ± SD, and were analyzed by 2-way ANOVA. Significant 2-way interactions were noted for 1genotype × sex in weanling mice (P = .0053), and 2pregnancy × genotype (P < .0005) in adult female mice, in regards to total liver Fe levels. Because there were significant 2-way interactions, multiple pairwise comparisons were made by Tukey's HSD post-hoc test. Different letters to the left of values indicate statistically significant differences (P < .05). The following additional significant effects were noted: for adult mice, 3genotype main effect for liver iron (P = .0011), 4sex main effect for liver copper (P = .0021), and 5genotype main effect for serum copper (P = .0154); for neonatal mice, 6sex main effect for liver iron (P = .0164), 7genotype main effect for serum iron (P = .0006), and 8sex (P = .046) and 9genotype main effects for serum copper (P = .0173); for pregnant mice, 10pregnancy main effect for liver iron (P = .0006) and 11liver copper (P < .0001), and 12genotype (P = .003) and 13pregnancy (P < .0001) main effects for serum copper. 14Each group had 6 mice, except for those marked with * (which had 5 mice) and ** (which had 4 mice).