Synthetic EPO 15- or 16-mer peptides (with 10 residue overlaps) able to stimulate human TCLs
| Peptide number . | Peptide sequence . |
|---|---|
| 1 | APPRL ICDSR VLERY |
| 2 | ICDSR VLERY LLEAK |
| 3 | VLERY LLEAK EAENI |
| 4 | LLEAK EAENI TTGCA |
| 5 | EAENI TTGCA EHCSL |
| 6 | TTGCA EHCSL NENIT |
| 7 | EHCSL NENIT VPDTK |
| 8 | NENIT VPDTK VNFYA |
| 9 | VPDTK VNFYA WKRME |
| 10 | VNFYA WKRME VGQQA |
| 11 | WKRME VGQQA VEVWQ |
| 12 | VGQQA VEVWQ GLALL |
| 13 | VEVWQ GLALL SEAVL |
| 14 | GLALL SEAVL RGQAL |
| 15 | SEAVL RGQAL LVNSS |
| 16 | RGQAL LVNSS QPWEP |
| 17 | LVNSS QPWEP LQLHV |
| 18 | QPWEP LQLHV DKAVS |
| 19 | LQLHV DKAVS GLRSL |
| 20 | DKAVS GLRSL TTLLR |
| 21 | GLRSL TTLLR ALGAQ |
| 22 | TTLLR ALGAQ KEAIS |
| 23 | ALGAQ KEAIS PPDAA |
| 24 | KEAIS PPDAA SAAPL |
| 25 | PPDAA SAAPL RTITA |
| 26 | SAAPL RTITA DTFRK |
| 27 | RTITA DTFRK LFRVY |
| 28 | DTFRK LFRVY SNFLR |
| 29 | LFRVY SNFLR GKLKL |
| 30 | SNFLR GKLKL YTGEA |
| 31 | GKLKL YTGEA CRTGD R |
| Peptide number . | Peptide sequence . |
|---|---|
| 1 | APPRL ICDSR VLERY |
| 2 | ICDSR VLERY LLEAK |
| 3 | VLERY LLEAK EAENI |
| 4 | LLEAK EAENI TTGCA |
| 5 | EAENI TTGCA EHCSL |
| 6 | TTGCA EHCSL NENIT |
| 7 | EHCSL NENIT VPDTK |
| 8 | NENIT VPDTK VNFYA |
| 9 | VPDTK VNFYA WKRME |
| 10 | VNFYA WKRME VGQQA |
| 11 | WKRME VGQQA VEVWQ |
| 12 | VGQQA VEVWQ GLALL |
| 13 | VEVWQ GLALL SEAVL |
| 14 | GLALL SEAVL RGQAL |
| 15 | SEAVL RGQAL LVNSS |
| 16 | RGQAL LVNSS QPWEP |
| 17 | LVNSS QPWEP LQLHV |
| 18 | QPWEP LQLHV DKAVS |
| 19 | LQLHV DKAVS GLRSL |
| 20 | DKAVS GLRSL TTLLR |
| 21 | GLRSL TTLLR ALGAQ |
| 22 | TTLLR ALGAQ KEAIS |
| 23 | ALGAQ KEAIS PPDAA |
| 24 | KEAIS PPDAA SAAPL |
| 25 | PPDAA SAAPL RTITA |
| 26 | SAAPL RTITA DTFRK |
| 27 | RTITA DTFRK LFRVY |
| 28 | DTFRK LFRVY SNFLR |
| 29 | LFRVY SNFLR GKLKL |
| 30 | SNFLR GKLKL YTGEA |
| 31 | GKLKL YTGEA CRTGD R |
Amino acid sequence (single letter code) of rhEPO (contains 166 amino acid residues) with bold residues indicating the main immunogenic epitopes identified in this study: APPRL ICDSR VLERY LLEAK EAENI TTGCA EHCSL NENIT VPDTK VNFYA WKRME VGQQA VEVWQ GLALL SEAVL RGQAL LVNSS QPWEP LQLHV DKAVS GLRSL TTLLR ALGAQ KEAIS PPDAA SAAPL RTITA DTFRK LFRVY SNFLR GKLKL YTGEA CRTGD R.