Antibodies and stains used for flow cytometry
| Antibody or probe . | Vendor . | Dilution . | Laser (nm) . | Dichroic . | Filter (nm) . |
|---|---|---|---|---|---|
| Sca1 APC | eBioscience | 1/200 | 641 | — | 670/30 |
| c-Kit FITC | BD Biosciences | 1/50 | 488 | 505LP | 515/20 |
| CD34 eFluor 450 | eBioscience | 1/50 | 407 | — | 450/50 |
| CD16/CD32 PE | eBioscience | 1/200 | 561 | — | 582/15 |
| CD135 PE | eBioscience | 1/50 | 561 | — | 582/15 |
| Lineage cocktail-biotin | |||||
| CD11b | BD Biosciences | 1/100 | N/A | N/A | N/A |
| CD8as | BD Biosciences | 1/200 | N/A | N/A | N/A |
| TER-119 | BD Biosciences | 1/400 | N/A | N/A | N/A |
| CD4 | BD Biosciences | 1/400 | N/A | N/A | N/A |
| CD45R | BD Biosciences | 1/400 | N/A | N/A | N/A |
| Ly-6G + Ly-6C | BD Biosciences | 1/25 | N/A | N/A | N/A |
| Streptavidin Alexa Fluor 700 | Life Technologies | 1/25 | 641 | 685LP | 730/45 |
| Live/dead blue stain | Life Technologies | 1/1000 | 355 | — | 450/50 |
| Antibody or probe . | Vendor . | Dilution . | Laser (nm) . | Dichroic . | Filter (nm) . |
|---|---|---|---|---|---|
| Sca1 APC | eBioscience | 1/200 | 641 | — | 670/30 |
| c-Kit FITC | BD Biosciences | 1/50 | 488 | 505LP | 515/20 |
| CD34 eFluor 450 | eBioscience | 1/50 | 407 | — | 450/50 |
| CD16/CD32 PE | eBioscience | 1/200 | 561 | — | 582/15 |
| CD135 PE | eBioscience | 1/50 | 561 | — | 582/15 |
| Lineage cocktail-biotin | |||||
| CD11b | BD Biosciences | 1/100 | N/A | N/A | N/A |
| CD8as | BD Biosciences | 1/200 | N/A | N/A | N/A |
| TER-119 | BD Biosciences | 1/400 | N/A | N/A | N/A |
| CD4 | BD Biosciences | 1/400 | N/A | N/A | N/A |
| CD45R | BD Biosciences | 1/400 | N/A | N/A | N/A |
| Ly-6G + Ly-6C | BD Biosciences | 1/25 | N/A | N/A | N/A |
| Streptavidin Alexa Fluor 700 | Life Technologies | 1/25 | 641 | 685LP | 730/45 |
| Live/dead blue stain | Life Technologies | 1/1000 | 355 | — | 450/50 |
APC, allophycocyanin; FITC, fluorescein isothiocyanate; N/A, not applicable.