Sequences of primers used in this study
| Purpose . | Forward primer (5′–3′) . | Reverse primer (5′–3′) . |
|---|---|---|
| ChIP–quantitative PCR | ||
| Myb promoter | CTCCCGGGTGTGTTGAAGTC | GCTCCCGAGCCCGAAT |
| Gata2 promoter | CTACCAGCCTCTTGCACAGCTA | GCCCCACCTCCACTGCTA |
| Luciferase activity | ||
| Myb promoter | CTCCCGGTACCGTTGAAGTCGGGGCGCAGTT | GTGTCACCCAACCCCATCGAGAGCTCCTTGAA |
| Mutant Myb promoter | GTTGAAGTCGGGGCGATGTAGAAACCCTGA AGG | CCTTCAGGGTTTCTACATCGCCCCGACTTCAAC |
| Gata2 promoter | GATAGAGGTACCGAGAGTTTGGGGAGTCAGTT | CCGTAGCAGTGGAGGTGGGGGAGCTCCTCCGC |
| Mutant Gata2 promoter | GAGAGTTTGGGGAGTATGTAGGATTTGGGCTGG | CCAGCCCAAATCCTACATACTCCCCAAACTCTC |
| Endothelial-specific genes | ||
| Cdh5 | ACCGCTGATCGGCACTGT | TCGGAAGAATTGGCCTCTGT |
| Tek | GATACAGCCTTTCCCATCCTAATCT | CAGAAGCAGGCGGTAACAGTCT |
| Purpose . | Forward primer (5′–3′) . | Reverse primer (5′–3′) . |
|---|---|---|
| ChIP–quantitative PCR | ||
| Myb promoter | CTCCCGGGTGTGTTGAAGTC | GCTCCCGAGCCCGAAT |
| Gata2 promoter | CTACCAGCCTCTTGCACAGCTA | GCCCCACCTCCACTGCTA |
| Luciferase activity | ||
| Myb promoter | CTCCCGGTACCGTTGAAGTCGGGGCGCAGTT | GTGTCACCCAACCCCATCGAGAGCTCCTTGAA |
| Mutant Myb promoter | GTTGAAGTCGGGGCGATGTAGAAACCCTGA AGG | CCTTCAGGGTTTCTACATCGCCCCGACTTCAAC |
| Gata2 promoter | GATAGAGGTACCGAGAGTTTGGGGAGTCAGTT | CCGTAGCAGTGGAGGTGGGGGAGCTCCTCCGC |
| Mutant Gata2 promoter | GAGAGTTTGGGGAGTATGTAGGATTTGGGCTGG | CCAGCCCAAATCCTACATACTCCCCAAACTCTC |
| Endothelial-specific genes | ||
| Cdh5 | ACCGCTGATCGGCACTGT | TCGGAAGAATTGGCCTCTGT |
| Tek | GATACAGCCTTTCCCATCCTAATCT | CAGAAGCAGGCGGTAACAGTCT |