Prothrombinase inhibition by peptide variants
| Peptide . | IC50, µM (95% CI)* . | Kd (nM ± SEM)† . |
|---|---|---|
| LIKTKRKRKK | 1.0 (0.4-2.7) | 6.0 ± 2.5 |
| AAKAKRKRKK | >308‡ | 5.9 ± 2.4 |
| AIKTKRKRKK | 70 (60-82) | 10.8 ± 3.9 |
| LAKTKRKRKK | 17 (7.7-36) | 16.2 ± 6.5 |
| LIKAKRKRKK | 110 (89-130) | 9.3 ± 3.6 |
| Peptide . | IC50, µM (95% CI)* . | Kd (nM ± SEM)† . |
|---|---|---|
| LIKTKRKRKK | 1.0 (0.4-2.7) | 6.0 ± 2.5 |
| AAKAKRKRKK | >308‡ | 5.9 ± 2.4 |
| AIKTKRKRKK | 70 (60-82) | 10.8 ± 3.9 |
| LAKTKRKRKK | 17 (7.7-36) | 16.2 ± 6.5 |
| LIKAKRKRKK | 110 (89-130) | 9.3 ± 3.6 |
CI, confidence interval; SEM, standard error of the mean.
IC50 values determined by fitting the data presented in Figures 4 and 5 to a 4-parameter variable slope equation using GraphPad Prism v.6.
Binding affinities determined by fitting the data presented in Figure 6A, as described by Marchand et al.31
30% inhibition observed at 308 µM of peptide.