Kinetic parameters of PRP-mediated clot contraction in F13a+/+, F13a+/−, and F13a−/− mice
| Platelets (×109/L) . | F13a+/+ . | F13a+/− . | F13a−/− . |
|---|---|---|---|
| Onset time, min | |||
| 400 | 5.2 ± 0.9 | 7.1 ± 0.3 | 6.4 ± 1.5 |
| 200 | 8.0 ± 1.6 | 7.8 ± 1.7 | 10.3 ± 1.6 |
| 50 | 17.6 ± 1.6†† | 17.8 ± 4.4 | 19.8 ± 5.5 |
| 10 | 22.2 ± 3.2††† | 22.3 ± 4.5† | 28.5 ± 4.6†† |
| Rate, 10−3/min | |||
| 400 | 54.8 ± 1.4 | 55.7 ± 2.3 | 75.8 ± 5.1* |
| 200 | 33.6 ± 2.1††† | 37.1 ± 1.9† | 45.1 ± 1.1*† |
| 50 | 17.4 ± 3.2††† | 22.3 ± 0.2††† | 26.4 ± 3.4†† |
| 10 | 10.6 ± 1.1††† | 16.4 ± 6.1††† | 31.0 ± 13.0†† |
| Maximum contraction (% of initial clot area) | |||
| 400 | 6.4 ± 0.4 | 6.7 ± 0.1 | 6.1 ± 0.2 |
| 200 | 10.5 ± 1.4 | 8.8 ± 1.0 | 6.7 ± 0.6* |
| 50 | 24.0 ± 0.9†† | 26.9 ± 2.7†† | 18.1 ± 6.5 |
| 10 | 49.3 ± 5.6††† | 47.5 ± 6.0††† | 67.3 ± 7.3††† |
| Platelets (×109/L) . | F13a+/+ . | F13a+/− . | F13a−/− . |
|---|---|---|---|
| Onset time, min | |||
| 400 | 5.2 ± 0.9 | 7.1 ± 0.3 | 6.4 ± 1.5 |
| 200 | 8.0 ± 1.6 | 7.8 ± 1.7 | 10.3 ± 1.6 |
| 50 | 17.6 ± 1.6†† | 17.8 ± 4.4 | 19.8 ± 5.5 |
| 10 | 22.2 ± 3.2††† | 22.3 ± 4.5† | 28.5 ± 4.6†† |
| Rate, 10−3/min | |||
| 400 | 54.8 ± 1.4 | 55.7 ± 2.3 | 75.8 ± 5.1* |
| 200 | 33.6 ± 2.1††† | 37.1 ± 1.9† | 45.1 ± 1.1*† |
| 50 | 17.4 ± 3.2††† | 22.3 ± 0.2††† | 26.4 ± 3.4†† |
| 10 | 10.6 ± 1.1††† | 16.4 ± 6.1††† | 31.0 ± 13.0†† |
| Maximum contraction (% of initial clot area) | |||
| 400 | 6.4 ± 0.4 | 6.7 ± 0.1 | 6.1 ± 0.2 |
| 200 | 10.5 ± 1.4 | 8.8 ± 1.0 | 6.7 ± 0.6* |
| 50 | 24.0 ± 0.9†† | 26.9 ± 2.7†† | 18.1 ± 6.5 |
| 10 | 49.3 ± 5.6††† | 47.5 ± 6.0††† | 67.3 ± 7.3††† |
Clot contraction parameters were determined by a nonlinear regression curve fit with plateau followed by 1-phase decay. Data represent mean ± SEM and were analyzed by ANOVA with Dunnett’s post-hoc test (n = 4).
P < .05,
P < .005, and
P < .0001 between platelet count, compared to 400 × 109/L platelets.
P < .005 between genotypes compared to F13a+/+.