Expression of blood group antigens (FORS1, A, and B) after DNA transfection of the selected expression constructs into COS1(B3GALNT1) and HeLa(FUT2) cells analyzed by FACS
| . | . | . | Cells/antigen . | |||||
|---|---|---|---|---|---|---|---|---|
| . | . | . | COS1(B3GALNT1)/FORS1 antigen . | HeLa(FUT2)/A antigen . | HeLa(FUT2)/B antigen . | |||
| Gene name . | Tripeptide sequence . | Amino acid substitution . | Pos cell % . | MFI value . | Pos cell % . | MFI value . | Pos cell % . | MFI value . |
| M_GBGT1 | GlyGlyAla | — | 9.3 | 2462 | 0.1 | ND | 0.1 | ND |
| H_ABO-A | LeuGlyGly | — | 0.4 | ND | 4.0 | 6361 | 0.2 | ND |
| Met43Thr | 0.3 | ND | 9.6 | 5617 | 0.1 | ND | ||
| Met53Thr | 0.4 | ND | 10.1 | 6506 | 0.0 | ND | ||
| Met69Thr | 2.1 | 521 | 10.0 | 5558 | 0.0 | ND | ||
| Met69Ser | 1.9 | 218 | 8.0 | 9169 | 0.1 | ND | ||
| Met142Thr | 0.2 | ND | 7.6 | 9449 | 0.0 | ND | ||
| GlyGlyAla | — | 0.6 | 79 | 5.2 | 6811 | 9.9 | 1307 | |
| Met69Thr | 3.5 | 497 | 6.8 | 6730 | 10.6 | 1244 | ||
| MetGlyAla | — | 0.3 | ND | 0.5 | ND | 33.9 | 1493 | |
| Met69Thr | 0.2 | ND | 0.5 | ND | 42.0 | 1331 | ||
| M_ABO-AB | GlyGlyAla | — | 4.0 | 1158 | 4.0 | 14349 | 16.5 | 1696 |
| Empty vector | — | — | 0.3 | ND | 0.2 | ND | 0.2 | ND |
| . | . | . | Cells/antigen . | |||||
|---|---|---|---|---|---|---|---|---|
| . | . | . | COS1(B3GALNT1)/FORS1 antigen . | HeLa(FUT2)/A antigen . | HeLa(FUT2)/B antigen . | |||
| Gene name . | Tripeptide sequence . | Amino acid substitution . | Pos cell % . | MFI value . | Pos cell % . | MFI value . | Pos cell % . | MFI value . |
| M_GBGT1 | GlyGlyAla | — | 9.3 | 2462 | 0.1 | ND | 0.1 | ND |
| H_ABO-A | LeuGlyGly | — | 0.4 | ND | 4.0 | 6361 | 0.2 | ND |
| Met43Thr | 0.3 | ND | 9.6 | 5617 | 0.1 | ND | ||
| Met53Thr | 0.4 | ND | 10.1 | 6506 | 0.0 | ND | ||
| Met69Thr | 2.1 | 521 | 10.0 | 5558 | 0.0 | ND | ||
| Met69Ser | 1.9 | 218 | 8.0 | 9169 | 0.1 | ND | ||
| Met142Thr | 0.2 | ND | 7.6 | 9449 | 0.0 | ND | ||
| GlyGlyAla | — | 0.6 | 79 | 5.2 | 6811 | 9.9 | 1307 | |
| Met69Thr | 3.5 | 497 | 6.8 | 6730 | 10.6 | 1244 | ||
| MetGlyAla | — | 0.3 | ND | 0.5 | ND | 33.9 | 1493 | |
| Met69Thr | 0.2 | ND | 0.5 | ND | 42.0 | 1331 | ||
| M_ABO-AB | GlyGlyAla | — | 4.0 | 1158 | 4.0 | 14349 | 16.5 | 1696 |
| Empty vector | — | — | 0.3 | ND | 0.2 | ND | 0.2 | ND |
The original construct names, tripeptide sequences, and amino acid substitutions are shown in the 3 leftmost columns. The A, B, and FORS1 antigen-positive cell percentages and MFI values of the antigen-positive cell populations are shown in the 6 rightmost columns. The MFI values are shown for samples whose positive cell percentages surpassed the background level of 0.5%.
ND, not determined; Pos, positive.