IgH CDR3 of Tet2-deficient CD19+B220low B cells reveals CLL-like features
| Mouse . | Tag . | Tet2 genotype . | V . | D . | J . | CDR3 . | Length, nt . | D reading frame . | IP . |
|---|---|---|---|---|---|---|---|---|---|
| Tet2 ANO mice | C12 | ANO/WT | V1-9 | D-FL 16.2 | J1 | C AR SYYGYS WYFDV W | 13 | 3 | 6,14 |
| A | ANO/WT | V2-2 | D-FL 16.3 | J4 | C AR KTNEVNYYDMDY W | 14 | 1 | 4,72 | |
| Tet2 CD19-Cre mice | G212 | Flox/WT | V1-55 | D-FL 16.1 | J1 | C AR IYYGSSYWYFDV W | 14 | 3 | 6,14 |
| G203 | Flox/WT | V3-2 | D-SP2.2 | J4 | C ARYYDYDYYAMDY W | 13 | 3 | 4,15 | |
| G322 | Flox/WT | V5-2 | D-FL 16.1 | J4 | C ARLWYYVG YAMDY W | 13 | 3 | 6,14 | |
| G328 | Flox/WT | V3-6 | D-SP2.9 | J4 | C AR DDGYYGD YAMDY W | 14 | 3 | 3,99 | |
| G211 | Flox/flox | V3-6 | D-SP2.5 | J4 | C A NYGNYIS YAMDY W | 13 | 3 | 3,75 | |
| G200 | Flox/flox | V3-6 | D-SP2.2 | J4 | C S TCPYDYDGS YYAMDY W | 16 | 3 | 3,46 | |
| G635 | Flox/flox | V2-2 | D-FL 16.1 | J4 | C AR TPPYITTVVA PYAMDY W | 18 | 1 | 6,14 | |
| G630 | Flox/flox | V5-15 | D-FL 16.1 | J4 | C AR HGGS AMDY W | 10 | 3 | 7,17 | |
| G103 | Flox/flox | V10-1 | D-Q52 | J2 | C VR HDWDG FDY W | 10 | 3 | 4,71 | |
| G213 | Flox/flox | V5-9-1 | D-SP2.5 | J2 | C TR EYLGSTTVPTIL DY W | 16 | 1 | 4,44 |
| Mouse . | Tag . | Tet2 genotype . | V . | D . | J . | CDR3 . | Length, nt . | D reading frame . | IP . |
|---|---|---|---|---|---|---|---|---|---|
| Tet2 ANO mice | C12 | ANO/WT | V1-9 | D-FL 16.2 | J1 | C AR SYYGYS WYFDV W | 13 | 3 | 6,14 |
| A | ANO/WT | V2-2 | D-FL 16.3 | J4 | C AR KTNEVNYYDMDY W | 14 | 1 | 4,72 | |
| Tet2 CD19-Cre mice | G212 | Flox/WT | V1-55 | D-FL 16.1 | J1 | C AR IYYGSSYWYFDV W | 14 | 3 | 6,14 |
| G203 | Flox/WT | V3-2 | D-SP2.2 | J4 | C ARYYDYDYYAMDY W | 13 | 3 | 4,15 | |
| G322 | Flox/WT | V5-2 | D-FL 16.1 | J4 | C ARLWYYVG YAMDY W | 13 | 3 | 6,14 | |
| G328 | Flox/WT | V3-6 | D-SP2.9 | J4 | C AR DDGYYGD YAMDY W | 14 | 3 | 3,99 | |
| G211 | Flox/flox | V3-6 | D-SP2.5 | J4 | C A NYGNYIS YAMDY W | 13 | 3 | 3,75 | |
| G200 | Flox/flox | V3-6 | D-SP2.2 | J4 | C S TCPYDYDGS YYAMDY W | 16 | 3 | 3,46 | |
| G635 | Flox/flox | V2-2 | D-FL 16.1 | J4 | C AR TPPYITTVVA PYAMDY W | 18 | 1 | 6,14 | |
| G630 | Flox/flox | V5-15 | D-FL 16.1 | J4 | C AR HGGS AMDY W | 10 | 3 | 7,17 | |
| G103 | Flox/flox | V10-1 | D-Q52 | J2 | C VR HDWDG FDY W | 10 | 3 | 4,71 | |
| G213 | Flox/flox | V5-9-1 | D-SP2.5 | J2 | C TR EYLGSTTVPTIL DY W | 16 | 1 | 4,44 |
Sequence analysis of PCR products obtained from GeneScan analysis was down using the JH primer. Results obtained were subject to analysis using IMGT/Vquest and IgBlast software to define all V, D, and J genes, as well as the CDR3 sequence. Columns show the identified IgH-V, -D, and -J genes; the resulting CDR3 sequence, the CDR3 length; the D genes reading frame, and the isoelectric point (IP) of the CDR3. The background of the mice that developed the abnormal B-cell population is indicated. 2 abnormal populations heterozygous for Tet2 gene-trap model (Tet2LacZ ANO) are shown, as well as 4 heterozygous Tet2 floxed alleles (Tet2−/+) and 6 homozygous inactivated Tet2 (Tet2−/−) in CD19-Cre Tet2 background. The last 3 lines represent analysis of 3 abnormal Tet2 populations that have emerged in 3 secondary recipients engrafted from the same primary Tet2CD19-Cre+ mice. In the “CDR3” column, amino acid residues encoded by the V or J segments (underlined) are separated by spaces from those encoded by the D segment (in bold and underlined).