The expression of the indicated markers was analyzed by FACS on Mϕs isolated from ovarian tumor ascites and on monocytes cultured for 5 days without (MΦs) or with ovarian tumor ascites (TA-MΦs), IL-6 (IL-6-MΦs), LIF (LIF-MΦs), or M-CSF (M-CSF-MΦs). Results are expressed as mean fluorescence intensity (MFI) and are representative of 5 separate experiments. For CD1a and CD83*, MFI was not detected in any of the subsets. For CD80 and CD86, MFI was less than 10.

+++ indicates MFI greater than 60 and less than 400; +++++, MFI greater than 1000; ++++, MFI greater than 400 and less than 1000; +, MFI greater than 10 and less than 30; ++, MFI greater than 30 and less than 60; −, MFI not detected; and +/−, MFI less than 10.

Cells were further stimulated for 48 hours with LPS before FACS analysis.