Quantitative real-time PCR analysis of ID4 and PAX5 mRNA levels
| Sample* . | Fold change of ID4 expression† . | Fold change of PAX5 expression‡ . |
|---|---|---|
| 6120 | 20.97 | 61.39 |
| 16503 | 142.68 | 33.13 |
| Normal thyroid | 111.69 | ND |
| BCP-ALL patient without t(6;14) | 0.00 | ND |
| BCP-ALL patient without t(6;14) | 0.01 | ND |
| BCP-ALL patient with no PAX5 deletion | ND | 478.82 |
| BCP-ALL patient with no PAX5 deletion | ND | 869.07 |
| Normal bone marrow§ | 1.00 | 1.00 |
| Sample* . | Fold change of ID4 expression† . | Fold change of PAX5 expression‡ . |
|---|---|---|
| 6120 | 20.97 | 61.39 |
| 16503 | 142.68 | 33.13 |
| Normal thyroid | 111.69 | ND |
| BCP-ALL patient without t(6;14) | 0.00 | ND |
| BCP-ALL patient without t(6;14) | 0.01 | ND |
| BCP-ALL patient with no PAX5 deletion | ND | 478.82 |
| BCP-ALL patient with no PAX5 deletion | ND | 869.07 |
| Normal bone marrow§ | 1.00 | 1.00 |
ND indicates not done.
The endogenous control gene, B2M, was used to normalize the results of both patients and controls (Applied Biosystems).
Controls were 2 patients without t(6;14) and normal thyroid tissue (seen to highly express ID4 according to the GeneHub-GEPIS, http://www.rbvi.ucsf.edu/Research/genentech/genehub-gepis/index.html11 ).
Controls were 2 patients with no PAX5 deletion.
ID4 and PAX5 mRNA levels were compared with expression in normal bone marrow (BD Biosciences, Oxford, United Kingdom).