BM DCs express a range of complement components, regulators, and receptors and C3a is generated locally
| Complement . | Gene array (intensity) . | Real-timeRT-PCR . |
|---|---|---|
| Alternative pathway | ||
| C3 | + (700) | + |
| fB | + (900) | + |
| Poperdin | + (500) | + |
| Classical pathway | ||
| C1q | + (500) | + |
| C2 | − | − |
| C4 | − | − |
| Terminal pathway | ||
| C5 | N/A | − |
| C6 | − | − |
| C8 | − | − |
| C9 | − | − |
| Inhibitory regulators | ||
| DAF | − | − |
| MCP | − | − |
| CR1/2 | − | − |
| fH | − | − |
| Crry | + (160) | + |
| CD59 | + (70) | + |
| Receptors | ||
| CR3 | + (380) | + |
| CR4 | + (430) | + |
| C3aR | + (480) | + |
| C5aR | + (50) | + |
| Complement . | Gene array (intensity) . | Real-timeRT-PCR . |
|---|---|---|
| Alternative pathway | ||
| C3 | + (700) | + |
| fB | + (900) | + |
| Poperdin | + (500) | + |
| Classical pathway | ||
| C1q | + (500) | + |
| C2 | − | − |
| C4 | − | − |
| Terminal pathway | ||
| C5 | N/A | − |
| C6 | − | − |
| C8 | − | − |
| C9 | − | − |
| Inhibitory regulators | ||
| DAF | − | − |
| MCP | − | − |
| CR1/2 | − | − |
| fH | − | − |
| Crry | + (160) | + |
| CD59 | + (70) | + |
| Receptors | ||
| CR3 | + (380) | + |
| CR4 | + (430) | + |
| C3aR | + (480) | + |
| C5aR | + (50) | + |
BM DCs were prepared from WT C57BL/6 mice. mRNA arrays and real-time RT-quantitative PCR were performed in DCs with 24-hour LPS stimulation. mRNA microarrays were performed in DCs from 2 individual mice, and similar results were obtained. A representative intensity of array signals is shown in brackets.
N/A indicates C5 information not available; +, present; and −, not present.