Pain behavior analysis in transgenic mice with sickle cell disease
| Pain characteristic . | Device . | Response . | Sensory stimuli . |
|---|---|---|---|
| Thermal (heat) | Hargreaves’ apparatus | Paw withdrawal latency | Heat targeted to the plantar surface of the hind paw |
| Thermal (cold) | Cold plate | Paw withdrawal latency and frequency | Cold at 4°C |
| Mechanical | Von Frey monofilaments | Paw withdrawal frequency | Application of filament to the plantar surface of the hind paw |
| Deep tissue/musculoskeletal | Grip force meter | Grip force exerted | None |
| Facial expression | Mouse grimace scale | Orbital tightening, ear position, nose bulge, cheek bulge | None |
| Conditioned place preference | Conditioned place preference boxes | Time spent in specific chamber | None |
| Vocalization after-discharge | Pressure-zone microphone | Vocalization | None |
| Pain characteristic . | Device . | Response . | Sensory stimuli . |
|---|---|---|---|
| Thermal (heat) | Hargreaves’ apparatus | Paw withdrawal latency | Heat targeted to the plantar surface of the hind paw |
| Thermal (cold) | Cold plate | Paw withdrawal latency and frequency | Cold at 4°C |
| Mechanical | Von Frey monofilaments | Paw withdrawal frequency | Application of filament to the plantar surface of the hind paw |
| Deep tissue/musculoskeletal | Grip force meter | Grip force exerted | None |
| Facial expression | Mouse grimace scale | Orbital tightening, ear position, nose bulge, cheek bulge | None |
| Conditioned place preference | Conditioned place preference boxes | Time spent in specific chamber | None |
| Vocalization after-discharge | Pressure-zone microphone | Vocalization | None |