Table 3. Effect of estrogen and related... | American Society of Hematology

Table 3.

Effect of estrogen and related compounds on the activity of FAAH, NOS, 15-LOX, and PLD in HUVECs

Sample	FAAH activity, % control	NOS activity, % control	15-LOX activity, % control	PLD activity, % control
Control	100^3-150	100^3-151	100^3-152	100^3-153
+100 nM E2	20 ± 2	400 ± 40	320 ± 32	275 ± 28
+100 nM E2-BSA	22 ± 2	420 ± 42	330 ± 33	290 ± 29
+100 nM E2+100 nM TMX	105 ± 10^3-154	107 ± 10^3-154	100 ± 10^3-154	100 ± 10^3-154
+100 nM E2+100 nM ICI182780	21 ± 2	390 ± 39	315 ± 32	270 ± 27
+100 nM E2+2 μM SR141716	20 ± 2	340 ± 34	310 ± 31	260 ± 26
+100 nM E2+100 nM HU-210	20 ± 2	640 ± 64#	320 ± 32	280 ± 28
+100 nM E2+2 μM CBD	21 ± 2	390 ± 39	320 ± 32	270 ± 27
+100 nM E2+2 μM CAPS	20 ± 2	400 ± 40	320 ± 32	275 ± 28
+100 nM E2+400 μMl-NAME	22 ± 2	25 ± 3^3-154	300 ± 30	280 ± 28
+100 nM E2+50 μM EGTA-am	20 ± 2	170 ± 17^3-154^**	150 ± 15^3-154^**	135 ± 13^3-154^**
+100 nM E2+10 μM ATFMK	5 ± 1^3-154	370 ± 37	280 ± 28	250 ± 25
+100 nM E2+10 μM ETYA	80 ± 8^3-154	390 ± 39	30 ± 3^3-154	260 ± 26
+100 nM E2+50 μM suramin	21 ± 2	400 ± 40	300 ± 30	52 ± 5^3-154^**
+100 nM E2+10 μM ST638	22 ± 2	390 ± 39	330 ± 33	60 ± 6^3-154^**

Sample	FAAH activity, % control	NOS activity, % control	15-LOX activity, % control	PLD activity, % control
Control	100^3-150	100^3-151	100^3-152	100^3-153
+100 nM E2	20 ± 2	400 ± 40	320 ± 32	275 ± 28
+100 nM E2-BSA	22 ± 2	420 ± 42	330 ± 33	290 ± 29
+100 nM E2+100 nM TMX	105 ± 10^3-154	107 ± 10^3-154	100 ± 10^3-154	100 ± 10^3-154
+100 nM E2+100 nM ICI182780	21 ± 2	390 ± 39	315 ± 32	270 ± 27
+100 nM E2+2 μM SR141716	20 ± 2	340 ± 34	310 ± 31	260 ± 26
+100 nM E2+100 nM HU-210	20 ± 2	640 ± 64#	320 ± 32	280 ± 28
+100 nM E2+2 μM CBD	21 ± 2	390 ± 39	320 ± 32	270 ± 27
+100 nM E2+2 μM CAPS	20 ± 2	400 ± 40	320 ± 32	275 ± 28
+100 nM E2+400 μMl-NAME	22 ± 2	25 ± 3^3-154	300 ± 30	280 ± 28
+100 nM E2+50 μM EGTA-am	20 ± 2	170 ± 17^3-154^**	150 ± 15^3-154^**	135 ± 13^3-154^**
+100 nM E2+10 μM ATFMK	5 ± 1^3-154	370 ± 37	280 ± 28	250 ± 25
+100 nM E2+10 μM ETYA	80 ± 8^3-154	390 ± 39	30 ± 3^3-154	260 ± 26
+100 nM E2+50 μM suramin	21 ± 2	400 ± 40	300 ± 30	52 ± 5^3-154^**
+100 nM E2+10 μM ST638	22 ± 2	390 ± 39	330 ± 33	60 ± 6^3-154^**

100% = 25 ± 3 pmol arachidonate × minute⁻¹ × mg protein⁻¹.

100% = 40 ± 4 pmol citrulline × minute⁻¹ × mg protein⁻¹.

100% = 370 ± 40 pmol 13-HODE × minute⁻¹ × mg protein⁻¹.

100% = 150 ± 15 pmol ethanolamine × minute⁻¹ × mg protein⁻¹;P < .01 and **P < .05 compared with control.

P < .01, compared with E2 (P > .05 in all other cases).

#P < .05 compared with E2 (P > .05 in all other cases).