Comparison of the human-based producer cell line Phoenix-GALV with the murine-based producer cell line PG13: transduction and engraftment of baboon marrow cells
| Animal . | Producer cell line . | % FACS+ on day 3 . | % EGFP/EYFP+ colonies . | Cells infused . | Days to ANC > 500 . | Follow-up (weeks) . |
|---|---|---|---|---|---|---|
| F99070 | Phoenix-GALV | 23.3 | 15.7 | 7.6 × 106/kg | 16 | 68 |
| PG13 | 9.2 | 7.8 | ||||
| 00021 | Phoenix-GALV | 20.8 | 24.8 | 12.6 × 106/kg | 11 | 65 |
| PG13 | 5.2 | 8.1 | ||||
| A00066 | Phoenix-GALV | 16.2 | 20.6 | 15.2 × 106/kg | 18 | 12* |
| PG13 | 6.4 | 4.4 |
| Animal . | Producer cell line . | % FACS+ on day 3 . | % EGFP/EYFP+ colonies . | Cells infused . | Days to ANC > 500 . | Follow-up (weeks) . |
|---|---|---|---|---|---|---|
| F99070 | Phoenix-GALV | 23.3 | 15.7 | 7.6 × 106/kg | 16 | 68 |
| PG13 | 9.2 | 7.8 | ||||
| 00021 | Phoenix-GALV | 20.8 | 24.8 | 12.6 × 106/kg | 11 | 65 |
| PG13 | 5.2 | 8.1 | ||||
| A00066 | Phoenix-GALV | 16.2 | 20.6 | 15.2 × 106/kg | 18 | 12* |
| PG13 | 6.4 | 4.4 |
All animals studied were transplanted as outlined in “Materials and methods” with oncoretroviral vectors pseudotyped by either Phoenix-GALV or PG13 as noted. Displayed are, for both vectors in each of the 3 animals, the percentage of fluorescence-positive cells in liquid culture 3 days after transduction and the percentage of fluorescence-positive colonies plated immediately after transduction. Growth factors used for this set of animals were stem cell factor, granulocyte colony-stimulating factor, and megakaryocyte growth and development factor at 100 ng/mL each.
Phoenix-GALV indicates Phoenix-GALV/MNDEYFPSN; PG13, PG13/MNDEGFPSN; EGFP, enhanced green fluorescent protein; EYFP, enhanced yellow fluorescent protein; and ANC, absolute neutrophil count.
Follow-up on this animal was discontinued and it was transferred to a different study.