Comparison of oncoretroviral vectors packaged by Phoenix-GALV with lentiviral vectors: transduction and engraftment of baboon marrow cells
| Animal . | Vector . | % FACS+ on day 3 . | % EGFP/EYFP+ colonies . | Cells Infused . | Days to ANC > 500 . | Follow-up (weeks) . |
|---|---|---|---|---|---|---|
| F99074 | Phoenix-GALV | 30.8 | nd | 14.8 × 106/kg | 19 | 27 |
| Lenti | 12.4 | nd | ||||
| F99310 | Phoenix-GALV | 35.0 | 37.3 | 30.9 × 106/kg | 12 | 25 |
| Lenti | 21.0 | 18.5 | ||||
| M99267 | Phoenix-GALV | 49.4 | 21.8 | 19.9 × 106/kg | 17 | 26 |
| Lenti | 22.0 | 13.4 |
| Animal . | Vector . | % FACS+ on day 3 . | % EGFP/EYFP+ colonies . | Cells Infused . | Days to ANC > 500 . | Follow-up (weeks) . |
|---|---|---|---|---|---|---|
| F99074 | Phoenix-GALV | 30.8 | nd | 14.8 × 106/kg | 19 | 27 |
| Lenti | 12.4 | nd | ||||
| F99310 | Phoenix-GALV | 35.0 | 37.3 | 30.9 × 106/kg | 12 | 25 |
| Lenti | 21.0 | 18.5 | ||||
| M99267 | Phoenix-GALV | 49.4 | 21.8 | 19.9 × 106/kg | 17 | 26 |
| Lenti | 22.0 | 13.4 |
All animals studied were transplanted as outlined in “Materials and methods” with oncoretroviral or lentiviral vectors as noted. Displayed are, for both vectors in each of the 3 animals, the percentage of fluorescence-positive cells in liquid culture 3 days after transduction and the percentage of fluorescence-positive colonies plated immediately after transduction. Growth factors used for this set of animals were interleukin 3, interleukin 6, stem cell factor, granulocyte colony-stimulating factor, megakaryocyte growth and development factor, and fms-like tyrosine kinase 3 ligand at 100 ng/mL each.
Phoenix-GALV indicates Phoenix-GALV/MNDEYFPSN; lenti, VSV-G/RRLsin.cPPT.hPGK.GFP.Wpre; EGFP, enhanced green fluorescent protein; EYFP, enhanced yellow fluorescent protein; ANC, absolute neutrophil count; and, nd, not done.