Quantitative analysis of VWF and fibrinogen deposition within thrombi generated under high shear rate conditions from blood of a healthy control or an AF patient
| . | Control . | AF . |
|---|---|---|
| VWF | 3.5 ± 1.2 × 103 | 2.0 ± 0.7 × 103 (μm3) |
| Fibrinogen | 1.3 ± 0.4 × 103 | 0 |
| . | Control . | AF . |
|---|---|---|
| VWF | 3.5 ± 1.2 × 103 | 2.0 ± 0.7 × 103 (μm3) |
| Fibrinogen | 1.3 ± 0.4 × 103 | 0 |
Experimental conditions were identical to those described in the Figure 5 legend (perfusion of whole blood without mepacrine labeling from healthy controls or an AF patient with a shear rate of 1500 s−1 for 7 minutes). Thrombi generated were fixed, double-stained with Cy3-labeled anti-VWF and Cy2-labeled antifibrinogen antibody, and viewed by CLSM. VWF- or fibrinogen-associated volume of individual thrombi was calculated based on images with a 1-μm interval from the collagen surface. Data represent the average ± SD of 5 individual thrombi randomly selected in a defined area (211 × 317 μm) in each single perfusion of blood from a control or patient, respectively.