Transcript levels of NF-κB/Rel-regulated antiapoptotic genes measured by real-time polymerase chain reaction
| Gene . | CD19+ B-cells (n = 6) median . | B-CLL (n = 27) median . | c-ALL lines (n = 3) median . |
|---|---|---|---|
| TRAF1 | 1.92 | 2.31 (P < .05) | 1.13 |
| TRAF2 | 1.54 | 1.52 | 1.64 |
| XIAP | 1.58 | 1.23 | 1.29 |
| Survivin | 1.12 | 0.91 | 3.45 |
| MnSOD | 3.32 (P < .05) | 1.83 | 2.35 |
| A20 | 6.13 (P < .05) | 2.35 | 2.13 |
| Bcl-xL | 1.53 | 1.53 | 2.45 |
| Bfl-1/A-1 | 2.81 (P < .05) | 1.92 | 0.99 |
| Gene . | CD19+ B-cells (n = 6) median . | B-CLL (n = 27) median . | c-ALL lines (n = 3) median . |
|---|---|---|---|
| TRAF1 | 1.92 | 2.31 (P < .05) | 1.13 |
| TRAF2 | 1.54 | 1.52 | 1.64 |
| XIAP | 1.58 | 1.23 | 1.29 |
| Survivin | 1.12 | 0.91 | 3.45 |
| MnSOD | 3.32 (P < .05) | 1.83 | 2.35 |
| A20 | 6.13 (P < .05) | 2.35 | 2.13 |
| Bcl-xL | 1.53 | 1.53 | 2.45 |
| Bfl-1/A-1 | 2.81 (P < .05) | 1.92 | 0.99 |
mRNA expression of NF-κB/Rel-regulated genes with antiapoptotic properties was measured by real-time polymerase chain reaction (PCR). Medians from normal CD19+ cells, B-CLL lymphocytes, and B-precursor cell lines are shown. Values are given as 10/Δct ratios. High ratios correspond to high expression and vice versa. TRAF1 is overexpressed in B-CLL cells compared to normal B cells (P < .05) and B-precursor cell lines. MnSOD, Bfl-1/A-1, and A20 were more strongly expressed in normal B cells (P < .05) than in B-CLL.