Characteristics of recipients of BCR-ABL–transduced BM cells transplanted immediately after retroviral gene transfer (nos. 1-11) or after 1 week (nos. 1A-14A) of culture in vitro
| Mouse ID . | Cell dose, × 104* . | Day mouse killed . | Spleen size, g† . | Pulmonary hemorrhage . | Liver infiltration . | Reconstitution, % Ly5.1 BM-SPL-THY . | Reconstitution, % GFP BM-SPL-THY . |
|---|---|---|---|---|---|---|---|
| BMT immediately afterBCR-ABL gene transfer | |||||||
| 1 | 20 | 10 | 0.46 | ND | Yes | ND | 45-49-4 |
| 2 | 20 | 10 | 0.38 | ND | Yes | ND | 39-49-5 |
| 3 | 20 | 10 | 0.48 | ND | Yes | ND | 44-47-1 |
| 4 | 20 | 10 | 0.48 | ND | Yes | ND | 45-48-1 |
| 5 | 20 | 10 | 0.44 | ND | Yes | ND | 41-41-1 |
| 6 | 20 | 11 | 0.28 | Yes | Yes | ND | ND |
| 7 | 20 | 11 | 0.29 | Yes | Yes | ND | ND |
| 8 | 20 | 12 | 0.33 | Yes | Yes | ND | ND |
| 9 | 20 | 12 | 0.21 | Yes | Yes | ND | ND |
| 10 | 20 | 11 | 0.48 | Yes | Yes | ND | 71-77-ND |
| 11 | 20 | 12 | 0.66 | Yes | Yes | ND | 70-68-ND |
| BMT 7 days after BCR-ABL gene transfer | |||||||
| 1A | 500 | 106 | 0.1 | No | No | ND | ND |
| 2A | 200 | 175 | 0.17 | No | No | ND | 0-0-0 |
| 3A | 20 | 175 | 0.16‡ | No | No | ND | 0-0-0 |
| 4A | 20 | 175 | 0.19 | No | No | ND | 0-1-0 |
| 5A | 20 | 52 | 0.12 | No | No | ND | 1-9-75 |
| 6A | 20 | 52 | 0.08 | No | No | ND | 0-0-2 |
| 7A | 20 | 52 | 0.12 | No | No | ND | 0-0-11-153 |
| 8A | 2 | 97 | 0.07 | No | No | 44-68-81 | 0-0-0 |
| 9A | 0.2 | 96 | 0.04 | No | No | 78-99-97 | 3-1-5 |
| 10A | 0.2 | 96 | 0.07 | No | No | 63-99-96 | 2-1-1 |
| 11A | 0.2 | 96 | 0.08 | No | No | 79-98-99 | 3-1-0 |
| 12A | 0.02 | 97 | 0.07 | No | No | 1-2-0 | ND |
| 13A | 0.02 | 117 | 0.06 | No | No | 2-2-1 | ND |
| 14A | 0.02 | 117 | 0.1 | No | No | 3-2-0 | ND |
| Mouse ID . | Cell dose, × 104* . | Day mouse killed . | Spleen size, g† . | Pulmonary hemorrhage . | Liver infiltration . | Reconstitution, % Ly5.1 BM-SPL-THY . | Reconstitution, % GFP BM-SPL-THY . |
|---|---|---|---|---|---|---|---|
| BMT immediately afterBCR-ABL gene transfer | |||||||
| 1 | 20 | 10 | 0.46 | ND | Yes | ND | 45-49-4 |
| 2 | 20 | 10 | 0.38 | ND | Yes | ND | 39-49-5 |
| 3 | 20 | 10 | 0.48 | ND | Yes | ND | 44-47-1 |
| 4 | 20 | 10 | 0.48 | ND | Yes | ND | 45-48-1 |
| 5 | 20 | 10 | 0.44 | ND | Yes | ND | 41-41-1 |
| 6 | 20 | 11 | 0.28 | Yes | Yes | ND | ND |
| 7 | 20 | 11 | 0.29 | Yes | Yes | ND | ND |
| 8 | 20 | 12 | 0.33 | Yes | Yes | ND | ND |
| 9 | 20 | 12 | 0.21 | Yes | Yes | ND | ND |
| 10 | 20 | 11 | 0.48 | Yes | Yes | ND | 71-77-ND |
| 11 | 20 | 12 | 0.66 | Yes | Yes | ND | 70-68-ND |
| BMT 7 days after BCR-ABL gene transfer | |||||||
| 1A | 500 | 106 | 0.1 | No | No | ND | ND |
| 2A | 200 | 175 | 0.17 | No | No | ND | 0-0-0 |
| 3A | 20 | 175 | 0.16‡ | No | No | ND | 0-0-0 |
| 4A | 20 | 175 | 0.19 | No | No | ND | 0-1-0 |
| 5A | 20 | 52 | 0.12 | No | No | ND | 1-9-75 |
| 6A | 20 | 52 | 0.08 | No | No | ND | 0-0-2 |
| 7A | 20 | 52 | 0.12 | No | No | ND | 0-0-11-153 |
| 8A | 2 | 97 | 0.07 | No | No | 44-68-81 | 0-0-0 |
| 9A | 0.2 | 96 | 0.04 | No | No | 78-99-97 | 3-1-5 |
| 10A | 0.2 | 96 | 0.07 | No | No | 63-99-96 | 2-1-1 |
| 11A | 0.2 | 96 | 0.08 | No | No | 79-98-99 | 3-1-0 |
| 12A | 0.02 | 97 | 0.07 | No | No | 1-2-0 | ND |
| 13A | 0.02 | 117 | 0.06 | No | No | 2-2-1 | ND |
| 14A | 0.02 | 117 | 0.1 | No | No | 3-2-0 | ND |
ID indicates identification; THY, thymus; BMT, bone marrow transplantation; and ND, not done.
Cell dose adjusted to day 0, that is, independent of the expansion during the 7-day culture. BCR-ABL–transduced cells expand by 30-fold in 7 days under the condition used (ie, these cultures were initiated with 2 × 105 cells per culture).
Normal size for adult mice is approximately 0.1 g.
Spleen was infiltrated by immature myeloid cells in this mouse.
Repopulation by BCR-ABL–transduced cells was robust in the thymus of this mouse (see mouse 7A in Figure 1G), indicating that EGFP was poorly expressed from the integrated provirus (Figure 1H).