Summary of the vascular bed–specific expression patterns of Hprt-targeted promoters
| Organ . | Promoter . | ||||
|---|---|---|---|---|---|
| VWF . | Flt-1 . | eNOS11 . | T112 . | T512 . | |
| Brain | ++s,l | +l | ++s,l | +l | +l |
| Heart | ++s | ++s,e | +s,l | − | +s,l |
| Lung | − | +s,l | +l | − | ++s,l |
| Kidney | − | ++s,l | +l | +s | +s,l |
| Spleen | − | ++s | +l | − | +s |
| Liver | − | − | − | − | +*l |
| Skeletal muscle | ++s | +s | +l | − | +s |
| Thymus | ++s | ++s | ND | ND | ND |
| Organ . | Promoter . | ||||
|---|---|---|---|---|---|
| VWF . | Flt-1 . | eNOS11 . | T112 . | T512 . | |
| Brain | ++s,l | +l | ++s,l | +l | +l |
| Heart | ++s | ++s,e | +s,l | − | +s,l |
| Lung | − | +s,l | +l | − | ++s,l |
| Kidney | − | ++s,l | +l | +s | +s,l |
| Spleen | − | ++s | +l | − | +s |
| Liver | − | − | − | − | +*l |
| Skeletal muscle | ++s | +s | +l | − | +s |
| Thymus | ++s | ++s | ND | ND | ND |
VWF indicates human promoter (5′ flanking region 2182 bp and first exon 246 bp and intron 1475 bp); Flt-1, human Flt-1 promoter (5′ flanking region 748 bp and first exon 284 bp); eNOS, human eNOS promoter (5′ flanking region 1600 bp); T1, mouse Tie-2 promoter (5′ flanking region 1760 bp and exon 318 bp); and T5, mouse Tie-2 promoter (5′ flanking region 1760 bp and first exon 318 bp and intron 1700 bp).
+ indicates patchy staining; ++, more uniform staining; −, no staining; s, predominant small vessel staining; l, predominant large vessel staining; e, ectopic staining; and ND, not done.
Unpublished observation (T.M., W.C.A., January 2002).