Table 2. Oligonucleotide primers used... | American Society of Hematology

Table 2.

Oligonucleotide primers used for PCR of the PIG-A gene

Amplified region	Sense primer		Antisense primer
Amplified region	Name	Primer sequence (5′-3′)	Name	Primer sequence (5′-3′)
Exon 2	mAIT1^*	GAGCTGAATTCTTGTTTTAC	A^*	GTATCACAAAGAGACACGG
Exon 2	mA1^*	CATTGCTCAGGTACCTATTTGTT	mBIT1^*	AAAGTCTAGAATGCAATTATAGC
Exon 3	mAIT2^*	AAGTGAATTCTCAGTCGTTCTGGT	mBIT2^*	CTTCTCCCTCGAGACAACATGAA
Exon 4, 5	mAIT3^*	ATGAAGCTTCACTCCTTTCTTCCCCTC	mBIT3^*	GCACTCGAGTACGTGAAACATCAAGTA
Exon 6	mAIT4^*	GGTCATTGTTTCTCGAGGGACAG	P12^*	ACCAGGTACCTCTTACAATCTAGGCTTCCTTC

Amplified region	Sense primer		Antisense primer
Amplified region	Name	Primer sequence (5′-3′)	Name	Primer sequence (5′-3′)
Exon 2	mAIT1^*	GAGCTGAATTCTTGTTTTAC	A^*	GTATCACAAAGAGACACGG
Exon 2	mA1^*	CATTGCTCAGGTACCTATTTGTT	mBIT1^*	AAAGTCTAGAATGCAATTATAGC
Exon 3	mAIT2^*	AAGTGAATTCTCAGTCGTTCTGGT	mBIT2^*	CTTCTCCCTCGAGACAACATGAA
Exon 4, 5	mAIT3^*	ATGAAGCTTCACTCCTTTCTTCCCCTC	mBIT3^*	GCACTCGAGTACGTGAAACATCAAGTA
Exon 6	mAIT4^*	GGTCATTGTTTCTCGAGGGACAG	P12^*	ACCAGGTACCTCTTACAATCTAGGCTTCCTTC

The primer sets amplify part or all of each exon and part of the intron flanking the exon.