Effect of N-Ras* on erythroid colony formation
| . | GFP . | N-Ras* (%) . |
|---|---|---|
| Colonies per 100 cells | 36 ± 6.0 | 23 ± 7.4† (63) |
| Average cells per colony | 1319 ± 612 | 720 ± 425† (55) |
| Clusters per 100 cells | 16 ± 9.5 | 27 ± 10.4† (170) |
| . | GFP . | N-Ras* (%) . |
|---|---|---|
| Colonies per 100 cells | 36 ± 6.0 | 23 ± 7.4† (63) |
| Average cells per colony | 1319 ± 612 | 720 ± 425† (55) |
| Clusters per 100 cells | 16 ± 9.5 | 27 ± 10.4† (170) |
Colony/cluster formation by day-3 GFP+, CD13− N-Ras*/control cells in the presence of IL-3, IL-6, SCF, and EPO with corresponding SD (n = 9). Colonies were analyzed on day 10. Significant difference of N-Ras
from control cultures (GFP alone) is indicated as follows: †P < .01. Percentages indicate percentage of control values.