Effect of N-Ras* on erythroid colony formation
| . | GFP . | N-Ras* (%) . |
|---|---|---|
| Day 8 cultures, colonies per 100 cells | 3.1 ± 5.4 | 0.9 ± 1.6 (29) |
| CD34+ from day 8 cultures, colonies per 100 cells | 35.2 ± 11.0 | 1.9 ± 2.7† (5) |
| . | GFP . | N-Ras* (%) . |
|---|---|---|
| Day 8 cultures, colonies per 100 cells | 3.1 ± 5.4 | 0.9 ± 1.6 (29) |
| CD34+ from day 8 cultures, colonies per 100 cells | 35.2 ± 11.0 | 1.9 ± 2.7† (5) |
Colony formation by day 8 cultures or CD34+ cells from day 8 cultures in the presence of IL-3, IL-6, SCF, and EPO with corresponding SD (n = 3). Colonies were analyzed on day 15; all colonies formed were gly A+, CD13−. Significant difference of N-Ras
from control cultures (GFP alone) is indicated as follows: †P < .01. Percentages indicate percentage of control values.